Glucose Uptake Assay Kit (Fluorometric) (ab136956)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Adherent cells, Suspension cells
- Sensitivity: 0.05 nmol/well
製品の概要
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製品名
Glucose Uptake Assay Kit (Fluorometric)
Glucose キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
検出感度
= 0.05 nmol/well -
全工程の試験時間
2h 00m -
種交差性
交差種: Mammals, Other species -
製品の概要
Glucose Uptake Assay Kit (Fluorometric) ab136956 is a highly sensitive and easy to use non-radioactive kit which can detect glucose uptake as low as 50 pmol/well in a variety of cell types.
2-deoxyglucose (2-DG) is used in glucose uptake assay protocols because of its structural similarity to glucose. 2-DG is taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P cannot be further metabolized, and thus accumulates within cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In this assay, the accumulated 2-DG6P is enzymatically oxidized and coupled to a probe, which generates fluorescence in the presence of NADPH.
Glucose uptake assay protocol summary:
- prepare cells with suitable glucose starvation / uptake stimulation depending on experimental set-up
- add 2-DG to cells and incubate for 20 mins at 37ºC
- wash cells with PBS to remove exogenous 2-DG
- lyse cells with extraction buffer and repeated pipetting
- freeze/thaw lysates and heat at 85ºC for 40 min
- cool on ice for 5 min
- add neutralizing buffer, spin and retain supernatant
- add supernatants and standards to wells
- add reaction mix and incubate for 40 min at 37ºC -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K666 Glucose Uptake Fluorometric Assay Kit. K666-100 is the same size as the 100 test size of ab136956.
If you want a more sensitive assay, we recommend using Glucose Uptake Assay Kit (Colorimetric) (ab136955), which contains an amplification step that allows the kit to detect < 10 pmol/well.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 100 tests 2-Deoxyglucose 1 x 1ml 1 x 1ml 2-DG Uptake Assay Buffer 1 x 10ml 1 x 10ml 2-DG6P Standard 1 vial 1 vial Enzyme Mix XXIV 1 vial 1 vial Extraction Buffer I 1 x 17ml 1 x 17ml Neutralizing Buffer 1 x 1ml 1 x 1ml PicoProbe II 1 x 0.2ml 1 x 0.2ml -
研究分野
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関連性
Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.
関連製品
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Related Products
画像
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Glucose Uptake measured in 3T3-L1 Adipocytes; I = Insulin.
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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2-DG6P Standard curve (a) and 2-DG uptake in Human adipocytes (b) , HeLa Cells (c) and 3T3-L1 cells (d) respectively. I=Insulin; P=Phloretin.
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Accumulated 2-DG6P is enzymatically oxidized and coupled to the picoprobe, which generates fluorescence in the presence of NADPH.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab136956 は 13 報の論文で使用されています。
- Liao W et al. Persistent high glucose induced EPB41L4A-AS1 inhibits glucose uptake via GCN5 mediating crotonylation and acetylation of histones and non-histones. Clin Transl Med 12:e699 (2022). PubMed: 35184403
- Williams SD et al. Hypoxia-Inducible Expression of Annexin A6 Enhances the Resistance of Triple-Negative Breast Cancer Cells to EGFR and AR Antagonists. Cells 11:N/A (2022). PubMed: 36230969
- Liu C et al. RBP4 Is Associated With Insulin Resistance in Hyperuricemia-Induced Rats and Patients With Hyperuricemia. Front Endocrinol (Lausanne) 12:653819 (2021). PubMed: 34177800
- Zhao X et al. circATP2B1 Promotes Aerobic Glycolysis in Gastric Cancer Cells Through Regulation of the miR-326 Gene Cluster. Front Oncol 11:628624 (2021). PubMed: 33996547
- Xu J et al. Dapagliflozin Restores Impaired Autophagy and Suppresses Inflammation in High Glucose-Treated HK-2 Cells. Cells 10:N/A (2021). PubMed: 34200774