Glucose Uptake Assay Kit (Colorimetric) (ab136955)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Adherent cells, Suspension cells
- Sensitivity: 0.01 nmol/well
製品の概要
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製品名
Glucose Uptake Assay Kit (Colorimetric)
Glucose uptake キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Cell-based (quantitative) -
検出感度
<= 0.01 nmol/well -
全工程の試験時間
3h 00m -
種交差性
交差種: Mammals, Other species -
製品の概要
Glucose Uptake Assay Kit (Colorimetric) (ab136955) is a highly sensitive and easy to use non-radioactive assay kit which can detect glucose uptake as low as 10 pmol/well in a variety of cell types.
2-deoxyglucose (2-DG) is used in glucose uptake assay protocols because of its structural similarity to glucose. 2-DG is taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P cannot be further metabolized, and thus accumulates within cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In this assay, the 2-DG6P is oxidized to generate NADPH, the level of which can be determined by an enzymatic recycling amplification reaction.
Glucose uptake assay protocol summary:
- prepare cells with suitable glucose starvation / uptake stimulation depending on experimental set-up
- add 2-DG to cells and incubate for 20 mins at 37ºC
- wash cells with PBS to remove exogenous 2-DG
- lyse cells with extraction buffer and repeated pipetting
- freeze/thaw lysates and heat at 85ºC for 40 min
- cool on ice for 5 min
- add neutralizing buffer, spin and transfer supernatant to new tubes
- add supernatants and standards to wells
- add reaction mix A and incubate for 1 hr at 37ºC
- add extraction buffer and heat to 90ºC for 40 min
- cool on ice for 5 min and add neutralizing buffer
- add reaction mix B
- analyze every 2-3 mins on microplate reader in kinetic mode at 37ºC
TEST -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K676 Glucose Uptake Colorimetric Assay Kit. K676-100 is the same size as the 100 test size of ab136955.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2-Deoxyglucose 1 x 1ml 2-DG6P Standard 1 vial Assay Buffer III 1 x 25ml Enzyme Mix III 1 vial Extraction Buffer I 1 x 17ml Glutathione Reductase 2 vials Neutralization Buffer II 1 x 2.5ml Recycling Mix 1 vial DTNB 2 vials -
研究分野
関連製品
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Related Products
画像
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2-DG6P Standard curve (a) and 2-DG uptake in 3T3-L1 cells (b), Human adipocytes (c) and HeLa cells (d) respectively. I=Insulin; P=Phloretin.
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Step A: 2-DG oxidation to generate NADPH; Step B: NADPH recycling amplification Reaction.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (159)
ab136955 は 159 報の論文で使用されています。
- Sun X et al. Glucose transporter GLUT1 expression is important for oriental river prawn (Macrobrachium nipponense) hemocyte adaptation to hypoxic conditions. J Biol Chem 299:102748 (2023). PubMed: 36436564
- Wang Q et al. YY1 transcription factor induces proliferation and aerobic glycolysis of neuroblastoma cells via LDHA regulation. Exp Ther Med 25:37 (2023). PubMed: 36569438
- Ding L et al. Canagliflozin primes antitumor immunity by triggering PD-L1 degradation in endocytic recycling. J Clin Invest 133:N/A (2023). PubMed: 36594471
- Wang YJ et al. Never-in-Mitosis A-Related Kinase 8 (NEK8) Regulates Adipogenesis, Glucose Homeostasis, and Obesity. Oxid Med Cell Longev 2022:1947067 (2022). PubMed: 36506932
- Li X et al. Long non-coding RNA CTSLP8 mediates ovarian cancer progression and chemotherapy resistance by modulating cellular glycolysis and regulating c-Myc expression through PKM2. Cell Biol Toxicol 38:1027-1045 (2022). PubMed: 34510316