製品の概要

  • 製品名

    Glucose Uptake Assay Kit (Cell-based)
    Glucose キット 製品一覧
  • 検出方法

    Fluorescent
  • サンプルの種類

    Adherent cells, Suspension cells
  • アッセイタイプ

    Cell-based
  • 全工程の試験時間

    0h 40m
  • 種交差性

    交差種: Other species, Mammals
  • 製品の概要

    Glucose Uptake Assay Kit (Cell-based) (ab204702) is an easy-to-use, non-radioactive kit that allows imaging and accurate measurement of glucose uptake in cultured cells in response to insulin and growth factors.


    The glucose uptake assay protocol is based on a fluorescent glucose analog, which just like glucose can be taken up by cells through glucose transporters. However, this glucose analog cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by the fluorescent glucose analog is proportional to the glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry.


    The kit includes phloretin, a natural phenol that inhibits glucose uptake, to use with control samples.


    Glucose uptake assay protocol summary:
    - remove cell culture media, and add glucose uptake mix to cells
    - incubate for 30 min at 37ºC
    - collect cells and keep on ice
    - wash with analysis buffer
    - analyze with flow cytometer or fluorescent microscope

  • 試験プラットフォーム

    Microplate reader, Fluor. microscope, Flow cyt.

製品の特性

画像

  • Comparison of histogram from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).

  • Images of Jurkat cells obtained using fluorescent microscope (Top: treated with phloretin; Bottom: without phloretin treatment).

  • Glucose Uptake in HeLa cells: HeLa cells showing the uptake of GluTracker Reagent in the cytoplasm. Cells were stained with GluTracker Reagent for 30 minutes and fixed. Image was taken using a fluorescent microscope with a 60X objective lens.

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参考文献

This product has been referenced in:

  • Valentín-Guillama G  et al. HIV-1 Envelope Protein gp120 Promotes Proliferation and the Activation of Glycolysis in Glioma Cell. Cancers (Basel) 10:N/A (2018). Read more (PubMed: 30200472) »
See 1 Publication for this product

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