Anti-Glucose Transporter GLUT4 (phospho S488) 抗体 [EPR930(2)] (ab188317)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)]
Glucose Transporter GLUT4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488) -
由来種
Rabbit -
アプリケーション
適用あり: WB, Dot blotmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Human fetal heart, mouse heart, rat heart
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR930(2) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab188317の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 55 kDa).
We recommend not to boil the samples before loading to get desirable WB results. |
Dot blot |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 55 kDa). We recommend not to boil the samples before loading to get desirable WB results. |
Dot blot
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Insulin-regulated facilitative glucose transporter. -
組織特異性
Skeletal and cardiac muscles; brown and white fat. -
関連疾患
Diabetes mellitus, non-insulin-dependent -
配列類似性
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
翻訳後修飾
Sumoylated. -
細胞内局在
Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. - Information by UniProt
-
参照データベース
- Entrez Gene: 6517 Human
- Entrez Gene: 20528 Mouse
- Entrez Gene: 25139 Rat
- Omim: 138190 Human
- SwissProt: P14672 Human
- SwissProt: P14142 Mouse
- SwissProt: P19357 Rat
- Unigene: 380691 Human
see all -
別名
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
see all
画像
-
All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution
Lane 1 : Human heart lysate, boiled
Lane 2 : Human heart lysate, unboiled
Lane 3 : Mouse heart lysate, boiled
Lane 4 : Mouse heart lysate, unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Lanes 1 and 2: 7 seconds exposure
Lanes 3 and 4: 20 seconds exposure
Blocking and diluting buffer: 5% NFDM/TBST
-
All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution
Lane 1 : Human fetal heart whole cell lysate
Lane 2 : Human fetal heart whole cell lysate with Phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugated (DC03L) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Diluting buffer and concentration 5% NFDM/TBST
-
Dot Blot analysis of Lane 1: Glucose Transporter GLUT4 (pS488) phospho peptide and Lane 2: Glucose Transporter GLUT4 non-phospho peptide labeling Glucose Transporter GLUT4 (phospho S488) with ab188317 at 1/1000 dilution.
5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
-
All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/5000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/20000 dilution
Lane 1 : Untreated human fetal heart
Lane 2 : Lambda phosphorylase treated human fetal heart
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (6)
ab188317 は 6 報の論文で使用されています。
- Zhang R et al. An NF-κB/OVOL2 circuit regulates glucose import and cell survival in non-small cell lung cancer. Cell Commun Signal 20:40 (2022). PubMed: 35346238
- Rahimi G et al. A combination of herbal compound (SPTC) along with exercise or metformin more efficiently alleviated diabetic complications through down-regulation of stress oxidative pathway upon activating Nrf2-Keap1 axis in AGE rich diet-induced type 2 diabetic mice. Nutr Metab (Lond) 18:14 (2021). PubMed: 33468193
- Montt-Guevara MM et al. D-Chiro-Inositol Regulates Insulin Signaling in Human Adipocytes. Front Endocrinol (Lausanne) 12:660815 (2021). PubMed: 33859622
- Zhuo J et al. Evaluation of type 2 diabetic mellitus animal models via interactions between insulin and mitogen-activated protein kinase signaling pathways induced by a high fat and sugar diet and streptozotocin. Mol Med Rep 17:5132-5142 (2018). PubMed: 29393432
- Wang N et al. Hydroxytyrosol ameliorates insulin resistance by modulating endoplasmic reticulum stress and prevents hepatic steatosis in diet-induced obesity mice. J Nutr Biochem 57:180-188 (2018). WB ; Mouse . PubMed: 29747118
- Liu Z et al. Adiponectin reduces ER stress-induced apoptosis through PPARa transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis 7:e2487 (2016). WB ; Mouse . PubMed: 27882945