Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell lines)
Loading amount
30 µg
Specification
breast cancer cell lines
Gel Running Conditions
Reduced Denaturing (10% gel + glycerol)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Concentration
0.5 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% milk
Secondary antibody
Name
Non-Abcam antibody was used: BioRad Goat anti-Rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 45-60 kDa
Positive control
U87 cell lysate
Negative control
unfortunately my experimental samples only weakly expressed Glut3, however positive control highly reactive.
Additional data
Additional Notes
Samples are NOT boiled-due to potential aggregation of Glut proteins.
Lane1: MDA-MB-231
Lane 2: MDA-MB-468
Lane 3: MCF-7
Lane 4: T47D
Lane 5: BT474
Lane 6: U87
Lane1: MDA-MB-231
Lane 2: MDA-MB-468
Lane 3: MCF-7
Lane 4: T47D
Lane 5: BT474
Lane 6: U87
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Kristina Terrell
Verified customer
投稿 Aug 20 2010