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Anti-Glucose Transporter GLUT3 抗体 (ab15311)

Reviews (4)Q&A (6)References (31)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)

Key features and details

  • Rabbit polyclonal to Glucose Transporter GLUT3
  • Suitable for: WB, ELISA, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Glucose Transporter GLUT3 antibody
    Glucose Transporter GLUT3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Glucose Transporter GLUT3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ELISA, IHC-Pmore details

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Synthetic peptide within Human Glucose Transporter GLUT3 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P11169

  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab15311の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P (2)
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Facilitative glucose transporter. Probably a neuronal glucose transporter.

  • 組織特異性

    Highly expressed in brain. Expressed in many tissues.

  • 配列類似性

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • brain antibody
      • FLJ90380 antibody
      • Glucose transporter type 3 antibody
      • Glucose transporter type 3 brain antibody
      • GLUT 3 antibody
      • GLUT-3 antibody
      • GLUT3 antibody
      • GTR3_HUMAN antibody
      • Slc2a3 antibody
      • Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 antibody
      • Solute carrier family 2, facilitated glucose transporter member 3 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)

      Formalin-fixed, paraffin-embedded human placenta tissue stained for Glucose Transporter GLUT3 using ab15311 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      ab15311 staining Glucose Transporter GLUT3 in human placenta by Immunohistochemistry (FFPE-sections).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)This image is courtesy of an anonymous Abreview.

      Formaldehyde-fixed, paraffin-embedded human testis tissue stained for Glucose Transporter GLUT3 using ab15311 at 1/200 dilution in immunohistochemical analysis.

      See Abreview

    参考文献 (31)

    ab15311 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab15311 は 31 報の論文で使用されています。

    • Crouse MS  et al. The effects of maternal nutrition during the first 50 d of gestation on the location and abundance of hexose and cationic amino acid transporters in beef heifer uteroplacental tissues. J Anim Sci 99:N/A (2021). PubMed: 33247721
    • Lauer V  et al. Hypoxia drives glucose transporter 3 expression through hypoxia-inducible transcription factor (HIF)-mediated induction of the long noncoding RNA NICI. J Biol Chem 295:4065-4078 (2020). PubMed: 31690629
    • Osumi H  et al. Tumor cell-derived angiopoietin-like protein 2 establishes a preference for glycolytic metabolism in lung cancer cells. Cancer Sci 111:1241-1253 (2020). PubMed: 32012400
    • James-Allan LB  et al. Changes in Placental Nutrient Transporter Protein Expression and Activity Across Gestation in Normal and Obese Women. Reprod Sci 27:1758-1769 (2020). PubMed: 32072607
    • Pilipenko V  et al. Neuroprotective potential of antihyperglycemic drug metformin in streptozocin-induced rat model of sporadic Alzheimer's disease. Eur J Pharmacol 881:173290 (2020). PubMed: 32580040
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 10 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT3 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC2 (pH6)
    Permeabilization
    No
    Specification
    Testis
    Blocking step
    Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 17 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Sample
    Human Tissue sections (Endometriosis)
    Specification
    Endometriosis
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Dr. Brett Mckinnon

    Verified customer

    投稿 Jun 10 2013

    Western blot abreview for Anti-Glucose Transporter GLUT3 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U87-MG, human brain, lymphoblastoid cell-line)
    Loading amount
    15 µg
    Specification
    U87-MG, human brain, lymphoblastoid cell-line
    Gel Running Conditions
    Reduced Denaturing (4-12 % Bis-Tris Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Mr. Georg Ziegler

    Verified customer

    投稿 Jan 11 2012

    Western blot abreview for Anti-Glucose Transporter GLUT3 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (breast cancer cell lines)
    Loading amount
    30 µg
    Specification
    breast cancer cell lines
    Gel Running Conditions
    Reduced Denaturing (10% gel + glycerol)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Miss. Kristina Terrell

    Verified customer

    投稿 Aug 20 2010

    Question

    Hi Thank you for your answer. Sadly, we have seen this exact pattern on 8 differents gels, with 20 sample, analysed at least twice. Our protocol are in french but briefly, we prepare cortex from rat brain on ice, homogenized them  in PBS 1X, spin 10 min 2500 rpm and resuspend in RIPA with anti-protease (cocktail sigma) Then, we did the western on a 12% separating gel, 4% stacking gel, we tried 20 ug, 50 ug and 100 ug of protein (as assayed by Bradford) We blocked overnight at 4 C with 5% milk in TBST (TBS + 1% tween 20) then incubate antibody GLUT3 (1:500) in TBST with 5% milk for 2h15, wahsing 3 times for 10 min then incubate with secondary antibody for 1h (1:10000 ). We used western lightning plus-ECL kit from perkin elmer to revelate. The protocol works for other antbody with the same sample (MCT-2, insulin receptor, b-actin). We also used the same membranes, stripped them and try with the other antibody and it was good. And GLUT-3 antibody also work with other kind of sample (hippocampus, dissected from the cortex of those same animals and prepared on the same day with the same solution as the total cortex). This is most strange. If you have any suggestion, we would be please to try something to resolve this

    Read More
    Answer

    Thank you for your reply and for these additional details.

    I can see no apparent reason why this product would be giving you this pattern with your rat cortex samples independent of age or treatment. It is especially strange to me that it is working correctly in hippocampal tissues from the same animals. Have you directly compared these hippocampal samples to the cortical samples?

    I look forward to your reply. Please let me know if you have any further questions.

    Read More

    Question

    Hi I have a question about GLUT-3 ab15311 antibody (lot 789020). In a western blot for 50 ug rat cortex, we see the expected band at 45-48 kDa but we also see a strong band at 38-40 kDa. Some rats have only this band, other have the 45 kDa and other have both (independant of the age or treatment of our rats). I saw in one of the abreviews that this band had been seen before but considered non specific. But in our case, it's so intense and clean, we don't know what to think of it.  We don't think glycosylation or phosphorylation will cause the band to be smaller (but we never know), as this been tested ? Thank you for your help.

    Read More
    Answer

    Thank you for contacting us.

    Your issue is very interesting as some animals because of the variablity between samples independent of age or treatment. Would you be able to provide your WB protocols and sample treatments? It may be an issue with the gel as the pattern looks similar to the image posted on our site but with lower weights, have you seen this response on multiple gels or just this once?



    Our products are covered by Abpromise guarantee for a period of six months after delivery. Should this product not perform as indicated on the datasheet, we provide scientific support, replacement or refund. More information on our Abpromise may be found at the following link:

    https://www.abcam.com/index.html?pageconfig=abpromise

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Ichh habe eine Frage bezüglich ab15311. Ich bekomme mit diesem GLUT3-Antikörper in all meinen humanen Lysaten eine klare Bande bei ca. 48 kDa und bin ziemlich sicher, dass diese Bande spezifisch ist für GLUT3, da auch in vielen anderen Publikationen GLUT3 in diesem Bereich gefunden wird und ich auch mit ab53095 und ab41525 die gleiche Bande bekomme. Meine diesbezüglichen Ergebnisse habe ich bereits als Abbreview auf Ihrer Seite veröffentlicht.
    Zusätzlich detektiert ab15311 jedoch eine teilweise noch stärkere Bande bei ca. 38 kDa. Diese Bande taucht nicht auf in humanen Hirnlysaten, humanen Spermatozoenlysaten und humanen Lymphozytenlysaten, allerdings in sämtlichen Lysaten, die aus kultivierten Zellen gewonnen wurden (EBV-LCLs, U87-MG, SHSY-5Y, Fibroblasten), in denen die GLUT3-Expression eher niedriger zu sein scheint.
    Nun meine Frage: ist es möglich, dass das Immunogen von ab15311 an ein Carrierprotein gekoppelt ist und daraus die Bande bei 38 kDa resultiert? Bei ab53095 bekomme ich interessanterweise neben der vermutlich spezifischen 48 kDa Bande eine zusätzliche Bande bei 29 kDa, was mich darin bestätigt, dass diese Banden unspezifisch sind. Es wäre von Vorteil, wenn Sie ein Blocking-Peptid für ab15311 zur Verfügung stellen könnten.

    Read More
    Answer

    Vielen Dank für Ihre Anfrage und für Ihre Geduld.

    Leider haben wir für die Antikörper ab15311 und ab53095 kein Blockpeptid, und können auch keines herstellen oder beschaffen, aber wir haben eines für den ab41525. Dieses hat die Katalognummer ab105625

    Click here (or use the following: https://www.abcam.com/Glucose-Transporter-GLUT3-peptide-ab105625.html).

    Als Carrier- Protein wurde KHL (Keyhole limpet hemocyanin) verwendet, welches laut BLAST keine Übereinstimmung mit der Immunogen- Sequenz aufweist. Wir sind daher genauso ratlos wie Sie bezüglich der Nebenbanden.

    Ich hoffe, dies hilft Ihnen trotzdem weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More

    Question

    We received 4 vials of antibody today that are all at room temperature. I am concerned that these antibodies will not work as expected and I don't want to waste time/effort/reagents with these suspect antibodies. I would like replacements of each as soon as possible.

    Read More
    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Sehr geehrte Damen und Herren,
    Bitte teilen Sie uns die IgG Konzentrationen nachfolgender Antikörper Lots mit.













    VEGFA

    abcam

    ab14994

    IgG1

    mouse

    mAb

    944661



    Glut3

    abcam

    ab15311

    IgG

    rabbit

    pAb

    GR-52148-1



    Glut3

    abcam

    ab15311

    IgG

    rabbit

    pAb

    GR62200-1




    Vielen Dank.


    Mit freundlichen Grüßen,

    Read More
    Answer

    Vielen Dank für Ihre Anfrage. Es freut mich bestätigen zu können, dass beide Antikörper keine Lot-spezifische Konzentration aufweisen. ab14994 hat eine Konzentration von 1mg/ml und ab15311 hat eine Konzentration von 0.2mg/ml. Bitte folgen Sie diesen links um zum jeweiligen datenblatt zugelangen und für mehr Informationen zu diesen Antikörpern: Click here (or use the following: https://www.abcam.com/VEGF165b-antibody-MRVL561-ab14994.html). Click here (or use the following: https://www.abcam.com/Glucose-Transporter-GLUT3-antibody-ab15311.html). Ich hoffe diese Information war hilfreich und wünsche Ihnen viel Erfolg mit Ihrer Forschung.

    Read More

    Question

    What is the homology between the immunogen of this antibody and the chicken sequence? The accession number of the chicken sequence is NP_990842.

    Read More
    Answer

    Thank you for your enquiry. I was able to obtain the immunogen sequence. This has been updated on the datasheet: SIEPAKETTTNV aa 485-496 of human Unfortunately, it appears there is no significant homology between the chicken sequence and the immunogen. I am sorry I could not be more helpful.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com