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Anti-Glucose Transporter GLUT1 抗体 (ab652)

  • Datasheet
  • SDS
Reviews (34)Q&A (28)References (175)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody (ab652)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody (ab652)

Key features and details

  • Rabbit polyclonal to Glucose Transporter GLUT1
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産
医薬用外毒物

製品の概要

  • 製品名

    Anti-Glucose Transporter GLUT1 antibody
    Glucose Transporter GLUT1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Glucose Transporter GLUT1
  • 由来種

    Rabbit
  • 特異性

    GLUT1.
  • アプリケーション

    適用あり: ICC/IFmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 478-492 of Human Glucose Transporter GLUT1 (Peptide available as ab115830.)

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.60
    Preservatives: 0.05% Sodium azide, 0.01% Thimerosal (merthiolate)
    Constituents: 0.164% Sodium phosphate, 1.45% Sodium chloride, 1.5% BSA
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

Our Abpromise guarantee covers the use of ab652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • 組織特異性

    Expressed at variable levels in many human tissues.
  • 関連疾患

    Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • 配列類似性

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在

    Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P11166 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 6513 Human
    • Entrez Gene: 20525 Mouse
    • Entrez Gene: 24778 Rat
    • Omim: 138140 Human
    • SwissProt: P11166 Human
    • SwissProt: P17809 Mouse
    • SwissProt: P11167 Rat
    • Unigene: 473721 Human
    • Unigene: 721551 Human
    • Unigene: 21002 Mouse
    • Unigene: 3205 Rat
    see all
  • 別名

    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody (ab652)
    Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody (ab652)
    ICC/IF image of ab652 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab652, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody (ab652)
    Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody (ab652)Image courtesy of an anonymous Abreview
    ab652 staining Glucose Transporter GLUT1 in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/250 for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

プロトコール

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (175)

    ab652 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab652 は 175 報の論文で使用されています。

    • Tran HB  et al. Enhanced inflammasome activation and reduced sphingosine-1 phosphate S1P signalling in a respiratory mucoobstructive disease model. J Inflamm (Lond) 17:16 (2020). PubMed: 32336954
    • Cartland SP  et al. Vascular transcriptome landscape of Trail-/- mice: Implications and therapeutic strategies for diabetic vascular disease. FASEB J N/A:N/A (2020). PubMed: 32501591
    • Zhu J  et al. Sevoflurane Induces Learning and Memory Impairment in Young Mice Through a Reduction in Neuronal Glucose Transporter 3. Cell Mol Neurobiol 40:879-895 (2020). PubMed: 31884568
    • Kuzyk CL  et al. Simvastatin Induces Delayed Apoptosis Through Disruption of Glycolysis and Mitochondrial Impairment in Neuroblastoma Cells. Clin Transl Sci 13:563-572 (2020). PubMed: 31917509
    • Natale BV  et al. ?9-tetrahydrocannabinol exposure during rat pregnancy leads to symmetrical fetal growth restriction and labyrinth-specific vascular defects in the placenta. Sci Rep 10:544 (2020). PubMed: 31953475
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 62 Abreviews or Q&A

    Western blot abreview for Anti-Glucose Transporter GLUT1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Mohammed Hossain

    Verified customer

    投稿 Nov 13 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Glucose Transporter GLUT1 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - 0.1% Triton-X
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 28 2020

    Western blot abreview for Anti-Glucose Transporter GLUT1 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Tissue lysate - other (Hypothalamus)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (10%gel)
    Loading amount
    30 µg
    Specification
    Hypothalamus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 28 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (renal carcinoma cell line xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    renal carcinoma cell line xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 09 2018

    Western blot abreview for Anti-Glucose Transporter GLUT1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse brain, liver, retina, muscle)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    25 µg
    Specification
    Mouse brain, liver, retina, muscle
    Blocking step
    Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 20 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (necrotic canine sarcoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
    Permeabilization
    No
    Specification
    necrotic canine sarcoma
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 18 2015

    Western blot abreview for Anti-Glucose Transporter GLUT1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (MCF7)
    Specification
    MCF7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 25 2015

    Question

    Where exactly does it bind to the transporter (extracellular side or cytoplasmic side)?

    Read More

    Abcam community

    Verified customer

    Asked on May 07 2014

    Answer

    The antibody ab652 binds to a cytoplasmic region of GLUT1.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on May 07 2014

    Question

    I would like to know the concentration of this antibody and also the appropriate negative control. for IHC (P). I want to use this for IHC on a mouse kidney specimen.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 21 2013

    Answer

    This antibody has not been purified and therefore its concentration has not been determined. It is a whole antiserum and so we estimate the concentration to be within the range of 1-10 mg/ml.
    As for the negative control, I would suggest using an isotype control as this is generally what is used as a negative control in IHC-P. For example, Rabbit IgG, monoclonal - Isotype Control (ab172730) or Rabbit IgG, monoclonal [SP137] - isotype control (ab125938) would be suitable.

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Aug 21 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum (goat) 1/50 diluted in PBS BSA5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: buffer citrate
    Sample
    Human Tissue sections (orbital fat)
    Specification
    orbital fat
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 19 2013

    1-10 of 62 Abreviews or Q&A

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