製品の概要

  • 製品名

    Glucose Oxidase Activity Assay Kit (Colorimetric)
    Glucose Oxidase キット 製品一覧
  • 検出方法

    Colorimetric
  • サンプルの種類

    Cell Lysate
  • アッセイタイプ

    Enzyme activity (quantitative)
  • 全工程の試験時間

    0h 20m
  • 種交差性

    交差種: Other species, Mammals
  • 製品の概要

    Glucose Oxidase Activity Assay Kit ab219924 uses a simple colorimetric assay to measure glucose oxidase (GOx) activity in cell and tissue extracts, and biological fluids. The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation or wash step.


    The glucose oxidase assay protocol uses a red probe to monitor glucose oxidase activity, leading to an increase in absorbance that can be easily detected at OD575 nm.


    Glucose oxidase assay protocol summary:
    - add samples and standards to wells
    - add glucose oxidase reaction mix
    - analyze every 2-3 mins with a microplate reader in kinetic mode for at least 10-30 mins at 37ºC

  • 特記事項

    Glucose oxidase (notatin, GOx, EC 1.1.3.4) is a dimeric enzyme that catalyzes oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.

  • 試験プラットフォーム

    Microplate reader

製品の特性

画像

  • Glucose Oxidase Activity Assay Kit (Colorimetric) (ab219924). Typical glucose oxidase dose response curve. Absorbance was measured on a 96-well clear bottom plate using a SpectraMax microplate reader with path check on. As low as 12.5 mU/mL glucose oxidase activity can be detected after 30 minutes incubation.

プロトコール

参考文献

ab219924 has not yet been referenced specifically in any publications.

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