製品の概要

  • 製品名

    Glucose Assay Kit - reducing agent compatible
    Glucose キット 製品一覧
  • 検出方法

    Colorimetric
  • サンプルの種類

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプ

    Quantitative
  • 検出感度

    > 0.02 mM
  • 検出範囲

    0.02 mM - 10 mM
  • 全工程の試験時間

    0h 30m
  • 製品の概要

    Glucose Assay Kit ab102517 provides direct measurement of glucose in biological samples. It is particularly suitable for serum and urine samples since it is unaffected by reducing substances, which can interfere with detection in oxidase-based kits. 


    In the glucose assay protocol, glucose is specifically oxidized to generate a product which reacts with a dye to generate color (λ = 450 nm) whose intensity is proportional to glucose concentration.


    The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes.


    The kit can detect glucose concentrations in the range of 20µM-10mM.


    Glucose assay protocol summary:
    - add reaction mix to sample and standard wells
    - incubate for 30 min
    - analyze with a microplate reader

  • 特記事項

    Previously called Glucose Detection Kit II

  • 試験プラットフォーム

    Microplate reader

製品の特性

画像

  • Glucose measured in biological fluids. Human samples diluted 20-80 fold. Mouse samples diluted 1-27 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Standard curve for glucose run using the kit protocol

プロトコール

参考文献

This product has been referenced in:

  • Feaver RE  et al. Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis. JCI Insight 1:e90954 (2016). Read more (PubMed: 27942596) »
  • Jou YC  et al. Foxp3 enhances HIF-1a target gene expression in human bladder cancer through decreasing its ubiquitin-proteasomal degradation. Oncotarget 7:65403-65417 (2016). Read more (PubMed: 27557492) »
See all 2 Publications for this product

レビューと Q&A

1-3 of 3 Abreviews or Q&A

Answer

The main difference between these two glucose assays is that in ab65333 the probe reacts with hydrogen peroxide and gets oxidized to produce colored compound detectable at 570nm or at Ex/Em 535/587nm. In ab102517, the probe reacts with NADH (produced during glucose dehydrogenation in the first step) and forms a colored product measured at 450nm.

Assay ab65333 can measure 1uM-10mM glucose. The additional sensitivity in ab65333 is achieved in the fluorometric measurement of the assay. Assay ab102517 can measure concentrations within the range of 20uM-10mM.

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Answer

Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm I would suggest Glucose Detection Kit ab102517or ab65333 Glucose Assay Kitswill be suitable for detection andquantification of glucose in the culture samples. I have put information below regarding the sensitivity and detection range of the kits, I can suggest to review this to decide which kit is best for your experiments (I am sorry we do not have concentration expressed as a percentage, I am not sure what this would be).

ab102517 has a senstivity of > 0.02 mM, range 0.02 mM - 10 mM
https://www.abcam.com/index.html?datasheet=102517 (or use the following: https://www.abcam.com/index.html?datasheet=102517).

ab65333 has a sensitivity of 1 µM, range 1 µM - 10000 µM
https://www.abcam.com/index.html?datasheet=65333 (or use the following: https://www.abcam.com/index.html?datasheet=65333).

I am sorry we do not sell directly to Russia. However, we do have an official distributor in Russia, Almabion. I can recommend to contact them directly to request a price quote:

ALMABION
ul. Vladimira Nevskogo d.59, k.166
Voronezh
394005
Russian Fed

Te l+7 4732 734828
Websitehttp://www.almabion.ru
Fax+7 4732 734828
mailto:Emailinfo@almabion.ru

I hope this will be helpful to you. Please do not hesitate to contact me if you have any further questions.

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Answer

Thakn you for your enquiry.
I am pleased to provide the further information below regarding the difference between these kits:


Measurement:

ab65333
Absorbance 570 nm or Fluorescence Ex/Em 535/587 nm

ab102517
Absorbance 450 nm

Uses:

ab65333
Serum, plasma, other body fluids, food, growth media, cell and tissue lysates

ab102517
Serum, plasma, other body fluids, food, growth media, cell and tissue lysates

Detection limit:

ab65333
1 uM glucose

ab102517
20 uM glucose

Advantages:

ab65333
More sensitive than ab102517

ab102517
Particularly suitable for serum and urine samples since it is unaffected by reducing substances which can interfere with other suppliers offering oxidase-based kits

We have a lot of ab65333 in stock. We currently have 2 of ab102517 in stock.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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