製品の概要

  • 製品名

    Glucose Assay Kit (Fluorometric, High Sensitivity)
    Glucose キット 製品一覧
  • 検出方法

    Fluorescent
  • サンプルの種類

    Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells, Tissue Culture Media
  • アッセイタイプ

    Quantitative
  • 検出感度

    < 0.5 µM
  • 種交差性

    交差種: Other species, Mammals
  • 製品の概要

    Picoprobe Glucose Assay Kit (ab169559) is a simple, rapid, ultra-sensitive assay to measure glucose. It is suitable for high-throughput use. In this assay, D-glucose is enzymatically oxidized to form a product which reacts with a colorless probe to generate the fluorescence (Ex/Em = 535/587 nm). The fluorescence generated is directly proportional to the amount of glucose. This assay kit can detect less than 0.5 µM glucose in various biological samples.

  • 特記事項

    Glucose is the main energy source for virtually all living organisms. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes.

  • 試験プラットフォーム

    Microplate reader

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 ラベル 100 tests
    Glucose Assay Buffer 1 x 25ml
    Glucose Enzyme Mix (lyophilized) Green 1 vial
    Glucose Standard Yellow 1 x 100µl
    Glucose Substrate Mix Red 1 vial
    PicoProbe Blue 1 x 0.4ml
  • 研究分野

  • 関連性

    Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.

画像

  • Representative standard curve.
  • Measurement of glucose levels in human serum (1 µL of 1:10 diluted) and rat tissue lysates from liver, kidney and muscle (0.14 µg, 0.19 µg and 0.93 µg respectively).

プロトコール

参考文献

This product has been referenced in:

  • Wang L  et al. Arylamine N-acetyltransferase 1 protects against reactive oxygen species during glucose starvation: Role in the regulation of p53 stability. PLoS One 13:e0193560 (2018). Read more (PubMed: 29518119) »
See 1 Publication for this product

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