Glucose and Sucrose Assay Kit (ab65334)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
製品の概要
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製品名
Glucose and Sucrose Assay Kit -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
アッセイタイプ
Quantitative -
全工程の試験時間
0h 40m -
種交差性
交差種: Mammals -
製品の概要
Abcam's Glucose and Sucrose Assay Kit provides a convenient means for measuring glucose and sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). To measure glucose level, glucose oxidase specifically oxidizes free-glucose generating a compound that reacts with the glucose probe to produce resorufin, which can be detected colorimetrically (OD570nm) or fluorometrically (Ex/Em 535/587). To measure sucrose, invertase can be added to the reaction to convert sucrose to free glucose and fructose, so total glucose level can be measured. Then the sucrose level = Total Glucose – Free Glucose.
Visit our FAQs page for tips and troubleshooting. -
特記事項
Glucose (C6H12O6; FW: 180.16) and sucrose (C12H22O11; FW:342.3) are the important fuel sources to generate universal energy molecule ATP. Measurement of glucose or sucrose level can be very important in both research and development process. Sucrose is a disaccharide which can be converted into one glucose and one fructose when adding Invertase.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Buffer II 1 x 25ml Development Enzyme Mix II 1 vial Invertase 1 vial OxiRed Probe 1 x 200µl Sucrose Standard 1 x 100µl -
研究分野
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関連性
Sucrose (C12H22O11; FW:342.3) is a disaccharide composed of glucose (C6H12O6; FW: 180.16) and fructose (C6H12O6; FW:180.16). Sucrose can be broken down by different In the human body, sucrose can be broken down by sucrase and/or invertase into its components, which are rapidly absorbed into the bloodstream. Once inside the cells, both monosaccharides will be processed and enter the glycolysis pathway to ultimately produce energy in form of ATP.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab65334 は 3 報の論文で使用されています。
- Jin ZL et al. Paeonol inhibits the progression of intracerebral haemorrhage by mediating the HOTAIR/UPF1/ACSL4 axis. ASN Neuro 13:17590914211010647 (2021). PubMed: 33906483
- Rodríguez D et al. Functional analysis of sucrase-isomaltase mutations from chronic lymphocytic leukemia patients. Hum Mol Genet N/A:N/A (2013). PubMed: 23418305
- Gerber LC et al. Incorporating microorganisms into polymer layers provides bioinspired functional living materials. Proc Natl Acad Sci U S A 109:90-4 (2012). PubMed: 22198770