Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6291] to Glucose 6 Phosphate Dehydrogenase - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Human
製品名Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291] - BSA and Azide free
Glucose 6 Phosphate Dehydrogenase 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6291] to Glucose 6 Phosphate Dehydrogenase - BSA and Azide free
アプリケーション適用あり: Flow Cyt, WBmore details
適用なし: ICC,IHC-P or IP
種交差性交差種: Mouse, Human
Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 100-200. The exact sequence is proprietary.
ab247989 is the carrier-free version of ab124738 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247989 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab247989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).|
機能Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
組織特異性Isoform Long is found in lymphoblasts, granulocytes and sperm.
パスウェイCarbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
関連疾患Anemia, non-spherocytic hemolytic, due to G6PD deficiency
配列類似性Belongs to the glucose-6-phosphate dehydrogenase family.
翻訳後修飾Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
- Information by UniProt
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291] (ab124738) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 59 kDa
This data was developed using ab124738, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.