Anti-Glucose 6 Phosphate Dehydrogenase 抗体 [EPR6291] (ab124738)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6291] to Glucose 6 Phosphate Dehydrogenase
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291]
Glucose 6 Phosphate Dehydrogenase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6291] to Glucose 6 Phosphate Dehydrogenase -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 100-200. The exact sequence is proprietary.
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ポジティブ・コントロール
- 293T, MOLT4, HUVEC and HeLa whole cell lysate (ab150035); Permeablized HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6291 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124738の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. -
組織特異性
Isoform Long is found in lymphoblasts, granulocytes and sperm. -
パスウェイ
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. -
関連疾患
Anemia, non-spherocytic hemolytic, due to G6PD deficiency -
配列類似性
Belongs to the glucose-6-phosphate dehydrogenase family. -
翻訳後修飾
Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. - Information by UniProt
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参照データベース
- Entrez Gene: 2539 Human
- Entrez Gene: 14381 Mouse
- Omim: 305900 Human
- SwissProt: P11413 Human
- SwissProt: Q00612 Mouse
- Unigene: 461047 Human
- Unigene: 684904 Human
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別名
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
see all
画像
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All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291] (ab124738) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 59 kDa -
ab124738 at 1/10 dilution staining Glucose 6 Phosphate Dehydrogenase in permeablized HeLa cells (red) or a rabbit IgG (negative) (green).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab124738 は 1 報の論文で使用されています。
- Bertola N et al. Mutated FANCA Gene Role in the Modulation of Energy Metabolism and Mitochondrial Dynamics in Head and Neck Squamous Cell Carcinoma. Cells 11:N/A (2022). PubMed: 35954197