Application
Western blot
Sample
Schizosaccharomyces pombe Cell lysate - other (whole cell lysate)
Loading amount
100 µg
Specification
whole cell lysate
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5
Secondary antibody
Name
Non-Abcam antibody was used: BD anti-rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Rabbit
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/2000
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: n/a kDa Non-specific: 10, 25, 43, 45 kDa
Positive control
Cerevisiae tca whole cell lysate
Additional data
Additional Notes
We did not see a specific band.
Abcam response
Thank you for your valuable feedback. S. pombe and S. cerevisiae are not tested species for ab993 and it seems that it is still unclear how specific this antibody is against glucose 6 phosphate dehydrogenase from these two species under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 02 2010