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Anti-Glucose 6 Phosphate Dehydrogenase 抗体 (ab993)

  • Datasheet
  • SDS
Reviews (12)Q&A (3)References (31)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
  • Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)

Key features and details

  • Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
  • Suitable for: IP, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

こちらの製品もご検討ください

一次抗体
Product image
Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
タンパク質
Product image
Recombinant human Glucose 6 Phosphate Dehydrogenase protein (ab126671)
タンパク質
Native Cow AGE-BSA protein (ab129535)

関連製品

製品の概要

  • 製品名

    Anti-Glucose 6 Phosphate Dehydrogenase antibody
    Glucose 6 Phosphate Dehydrogenase 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IP, WB, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 50-100. The exact sequence is proprietary.
    Database link: P11413

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

関連製品

  • Assay kits

    • Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529)
    • Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (ab176722)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant human Glucose 6 Phosphate Dehydrogenase protein (ab126671)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab993の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at 2-10 µg/mg of lysate.
WB (6)
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF (4)
Use a concentration of 5 µg/ml.
特記事項
IP
Use at 2-10 µg/mg of lysate.
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF
Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • 組織特異性

    Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • パスウェイ

    Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • 関連疾患

    Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • 配列類似性

    Belongs to the glucose-6-phosphate dehydrogenase family.
  • 翻訳後修飾

    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Target information above from: UniProt accession P11413 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2539 Human
    • Entrez Gene: 14381 Mouse
    • Omim: 305900 Human
    • SwissProt: P11413 Human
    • SwissProt: Q00612 Mouse
    • Unigene: 461047 Human
    • Unigene: 684904 Human
    • 別名

      • G6PD antibody
      • G6PD_HUMAN antibody
      • G6PD1 antibody
      • G6pdx antibody
      • Glucose 6 phosphate 1 dehydrogenase antibody
      • Glucose 6 phosphate dehydrogenase antibody
      • Glucose 6 phosphate dehydrogenase, G6PD antibody
      • Glucose-6-phosphate 1-dehydrogenase antibody
      • MET19 antibody
      • POS10 antibody
      • Zwf1p antibody
      see all

    画像

    • Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 59 kDa


      Exposure time: 30 seconds
    • Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)

      Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab993 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab993 at 1 µg/ml.

      Lane 1: ab993 (batch 3) IP in NIH/3T3 whole cell lysate.

      Lane 2: ab993 (batch 4) IP in NIH/3T3 whole cell lysate.

      Lane 3: Control IgG IP in NIH/3T3 whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

    プロトコール

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (31)

    ab993 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab993 は 31 報の論文で使用されています。

    • Wen J  et al. Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes. Sci Rep 10:2782 (2020). PubMed: 32066834
    • Liu J  et al. Ginsenoside Rh2 pretreatment and withdrawal reactivated the pentose phosphate pathway to ameliorate intracellular redox disturbance and promoted intratumoral penetration of adriamycin. Redox Biol 32:101452 (2020). PubMed: 32067911
    • Li Z  et al. APC-Cdh1 Regulates Neuronal Apoptosis Through Modulating Glycolysis and Pentose-Phosphate Pathway After Oxygen-Glucose Deprivation and Reperfusion. Cell Mol Neurobiol 39:123-135 (2019). PubMed: 30460429
    • Tao L  et al. Rev-erba inhibits proliferation by reducing glycolytic flux and pentose phosphate pathway in human gastric cancer cells. Oncogenesis 8:57 (2019). PubMed: 31591390
    • Min KH & Lee W Alteration of mitochondrial DNA content modulates antioxidant enzyme expressions and oxidative stress in myoblasts. Korean J Physiol Pharmacol 23:519-528 (2019). PubMed: 31680774
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 15 Abreviews or Q&A

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116 colon cancer cells)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    HCT116 colon cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Míriam Tarrado-Castellarnau

    Verified customer

    投稿 Oct 02 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.1% Triton X100
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 30 2017

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (Mouse brain)
    Specification
    Mouse brain
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 02 2014

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Mouse Tissue lysate - other (Lung cytosol)
    Specification
    Lung cytosol
    Blocking step
    (agent) for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Amit Agarwal

    Verified customer

    投稿 Jul 19 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (WBCs)
    Specification
    WBCs
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 11 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Leukocytes from bone marrow)
    Specification
    Leukocytes from bone marrow
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 28 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Bone marrow cells)
    Specification
    Bone marrow cells
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 09 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Rat testis)
    Specification
    Rat testis
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 01 2010

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Schizosaccharomyces pombe Cell lysate - other (whole cell lysate)
    Loading amount
    100 µg
    Specification
    whole cell lysate
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 02 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Skeletal muscle)
    Specification
    Skeletal muscle
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 19 2010

    1-10 of 15 Abreviews or Q&A

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