製品の概要

  • 製品名

    Glucose-1-Phosphate Assay Kit (Colorimetric)
  • 検出方法

    Colorimetric
  • サンプルの種類

    Tissue, Adherent cells, Suspension cells, Tissue Extracts
  • アッセイタイプ

    Quantitative
  • 検出感度

    = 1 µM
  • 検出範囲

    1 µM - 10000 µM
  • 全工程の試験時間

    0h 40m
  • 種交差性

    交差種: Other species, Mammals
  • 製品の概要

    In Glucose-1-phosphate Assay Kit (ab155892), G1P (glucose-1-phosphate) is converted to glucose-6-phosphate by phosphoglucomutase in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a colorless probe to a colored product with strong absorbance at 450 nm. Abcam's Glucose-1-phosphate Assay Kit is rapid, sensitive and easy to use and can detect 1µM - 10 mM G1P. This assay kit can be used for a variety of sample types.

  • 特記事項

    Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose metabolism and storage. In response to hormonal or neural signals, glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen through the actions of Phosphoglucose isomerase (G6P→ G1P), UDPG-pyrophosphorylase (G1P → UDP-glucose) and glycogen synthase (UDP-glucose + glycogen[n] → UDP + glycogen[n+1]). Glucose-1-phosphate is found in virtually all organisms from bacteria to higher plants and animals. Measurement of intracellular G1P levels is crucial for analyzing the carbohydrate metabolic pathways and their kinetic properties

  • 試験プラットフォーム

    Microplate reader

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 ラベル 100 tests
    G1P Assay Buffer WM 1 x 27ml
    G1P Developer Green 1 vial
    G1P Enzyme Mix Purple 1 vial
    G1P Standard Yellow 1 vial
    G1P Substrate Mix Red 1 vial
  • 研究分野

  • 別名

    • G1P

画像

  • Measurement of glucose-1-phosphate in jackfruit seed, bovine liver & jurkat cell lysate.
  • Typical Standard Curve

プロトコール

参考文献

ab155892 has not yet been referenced specifically in any publications.

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