Anti-Glucocorticoid Receptor beta 抗体 (ab233165)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor beta
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-Glucocorticoid Receptor beta antibody
Glucocorticoid Receptor beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Glucocorticoid Receptor beta -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, Pig
交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Orangutan, Squirrel monkey -
免疫原
Recombinant fragment corresponding to Human Glucocorticoid Receptor beta aa 550 to the C-terminus.
Database link: P04150 -
ポジティブ・コントロール
- WB: Recombinant human Glucocorticoid Receptor beta; Pig brain tissue lysate. IHC-P: Human lung cancer and stomach tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233165の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 86 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 86 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
ターゲット情報
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機能
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation. -
組織特異性
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. -
関連疾患
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻訳後修飾
Increased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2908 Human
- Entrez Gene: 14815 Mouse
- Entrez Gene: 24413 Rat
- SwissProt: P04150 Human
- SwissProt: P06537 Mouse
- SwissProt: P59667 Rabbit
- SwissProt: P06536 Rat
- Unigene: 122926 Human
see all -
別名
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
画像
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Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Glucocorticoid Receptor beta using ab233165 at 20μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for Glucocorticoid Receptor beta using ab233165 at 20μg/ml in immunohistochemical analysis. DAB staining.
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Anti-Glucocorticoid Receptor beta antibody (ab233165) at 2 µg/ml + Recombinant human Glucocorticoid Receptor beta
Secondary
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 86 kDa -
Anti-Glucocorticoid Receptor beta antibody (ab233165) at 2 µg/ml + Pig brain tissue lysate
Secondary
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 86 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab233165 は 2 報の論文で使用されています。
- Lo CY et al. Increased Interleukin-17 and Glucocorticoid Receptor-β Expression in Interstitial Lung Diseases and Corticosteroid Insensitivity. Front Immunol 13:905727 (2022). PubMed: 35865549
- Wang SB et al. Increased lipopolysaccharide content is positively correlated with glucocorticoid receptor-beta expression in chronic rhinosinusitis with nasal polyps. Immun Inflamm Dis 8:605-614 (2020). PubMed: 32870597