Application
Western blot
Sample
Pig Cell lysate - whole cell (Porcine brain endothelial cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
15 µg
Specification
Porcine brain endothelial cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Mouse-anti Rabbit (SC2357)
Host species: Mouse lemur
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse lemur
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 85 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Yu Siong Ho
Verified customer
投稿 Jul 31 2019