Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HELA)
Specification
HELA
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TRITON-X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: GOAT ANTI-RABBIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/2000
Additional data
Additional Notes
Not a very bright staining pattern could be nonspecific staining
Abcam response
We would like to thanks this user for the valuable feedback. According to the literature the Glucocorticoid Receptor is located in both the cytoplasm and the nucleus as seen in this image. We would like to hear from other users that used this antibody in ICC/IF.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 05 2011