Anti-Glucocorticoid Receptor 抗体 (ab3671)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human, Snake
- Isotype: IgG
製品の概要
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製品名
Anti-Glucocorticoid Receptor antibody
Glucocorticoid Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Glucocorticoid Receptor -
由来種
Rabbit -
特異性
This antibody detects both the unactivated and activated forms of GR. -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human, Snake -
免疫原
Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 150-175.
Sequence:APTEKEFPKTHSDVSSEQQHLKGQTG
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/250. | |
| ICC/IF | 1/250. | |
| WB | 1/500 - 1/2500. Detects a band of approximately 97 kDa (predicted molecular weight: 86 kDa). |
ターゲット情報
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機能
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation. -
組織特異性
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. -
関連疾患
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻訳後修飾
Increased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2908 Human
- Entrez Gene: 14815 Mouse
- Omim: 138040 Human
- SwissProt: P04150 Human
- SwissProt: P06537 Mouse
- Unigene: 122926 Human
- Unigene: 129481 Mouse
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別名
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
画像
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Western blot - Anti-Glucocorticoid Receptor antibody (ab3671)All lanes : Anti-Glucocorticoid Receptor antibody (ab3671) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) membrane enriched extract
Lane 2 : MCF7 (human breast adenocarcinoma cell line) membrane enriched extract
Lane 3 : T-47D membrane enriched extract
Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) membrane enriched extract
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) membrane enriched extract
Lane 6 : Mouse brain tissue extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunocytochemistry/Immunofluorescence analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial adenocarcinoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Thamnophis sirtalis (Common garter snake) brain tissue sections labeling Glucocorticoid Receptor with ab3671 at 1/250. Samples were blocked with 10% goat serum in 0.1M PBS. Samples were incubated with the primary antibody for 48 hours at 4°C. A biotin-conjugated goat anti-rabbit was used as the secondary antibody.
プロトコール
データシートおよび資料
参考文献 (2)
ab3671 は 2 報の論文で使用されています。
- Nettersheim D et al. Deciphering the molecular effects of romidepsin on germ cell tumours: DHRS2 is involved in cell cycle arrest but not apoptosis or induction of romidepsin effectors. J Cell Mol Med 23:670-679 (2019). PubMed: 30460772
- Gatta E et al. Genome-wide methylation in alcohol use disorder subjects: implications for an epigenetic regulation of the cortico-limbic glucocorticoid receptors (NR3C1). Mol Psychiatry N/A:N/A (2019). PubMed: 31239533
