Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (Expression of Human Glucocorticoid Receptor in CHO)
Permeabilization
Yes - 0.1 % Triton X-100
Specification
Expression of Human Glucocorticoid Receptor in CHO
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBS + 3% Dried Milk + 0.1 % Triton X-100
Dilution
1/100
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: goat anti rabbit FITC
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
The figure clearly shows the nuclear localization of GR in CHO K1 cells expressing human GR. The second picture shows a DAPI nuclear stain of the same cells to highlight the nucleus.
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投稿 Jul 29 2008