Anti-Glucocorticoid Receptor 抗体 (ab227524)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Glucocorticoid Receptor antibody
Glucocorticoid Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Glucocorticoid Receptor -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Sheep, Cow, Cat -
免疫原
Recombinant fragment within Human Glucocorticoid Receptor (internal sequence). The exact sequence is proprietary.
Database link: P04150 -
ポジティブ・コントロール
- WB: HeLa whole cell extract; Jurkat and Raji whole cell lysates. IP: HeLa whole cell extract. IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227524の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 86 kDa.
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IP |
1/500 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 86 kDa. |
IP
1/500 - 1/1000. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation. -
組織特異性
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. -
関連疾患
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻訳後修飾
Increased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2908 Human
- Omim: 138040 Human
- SwissProt: P04150 Human
- Unigene: 122926 Human
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別名
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
画像
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All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Glucocorticoid Receptor Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 86 kDa -
All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Glucocorticoid Receptor knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 86 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded human colon carcinoma tissue stained for Glucocorticoid Receptor using ab227524 at 1/250 dilution in immunohistochemical analysis.
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Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for Glucocorticoid Receptor (green) using ab227524 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Glucocorticoid Receptor was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2.5 µg of ab227524. Western blot was performed from the immunoprecipitate using ab227524 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 µg (Input).
Lane 2: 2.5 µg preimmune rabbit IgG instead of ab227524 in HeLa whole cell lysate.
Lane 3: ab227524 IP in HeLa whole cell lysate.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab227524 は論文での使用が確認できていません。