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Anti-Glucagon 抗体 [K79bB10] (ab10988)

Reviews (13)Q&A (6)References (140)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)

Key features and details

  • Mouse monoclonal [K79bB10] to Glucagon
  • Suitable for: IHC-P
  • Reacts with: Rat
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Glucagon antibody [K79bB10]
    Glucagon 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [K79bB10] to Glucagon

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Rat
    交差が予測される動物種: Rabbit, Guinea pig, Cat, Dog, Human, Pig, Common marmoset, Syrian hamster

  • 免疫原

    Full length protein corresponding to Pig Glucagon. Polymerized porcine glucagon.
    Database link: P01275

  • ポジティブ･コントロール

    • Pancreatic glucagon for RIA. IHC-P: Mouse and Rat pancreas tissues.

  • 特記事項

    Production of this antibody has been changed on 8th July 2016. The following lots are from ascites and are still in stock as of 8th July 2016: GR260160 and GR268340.  Lot numbers higher than GR268340 will be purified from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab10988の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (10)
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.

  • 細胞内局在

    Secreted

  • 参照データベース

    see all

  • 別名

    • GCG antibody

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)

    Formali-fixed, paraffin-embedded rat pancreas tissue stained for Glucagon using ab10988 at 10 µg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)Image courtesy of Thomas Schoeppe by Abreview.
    ab10988 staining Glucagon in rat pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde, blocked with 2% serum for 1 hour at 25°C, then incubated with ab10988 at a 1/200 dilution for 1 hour at 25°C. The secondary used was a donkey anti-mouse IgG (H+L) conjugated to Texas Red, used at a 1/250 dilution. Nuclei counterstained with DAPI, green is staining for insulin.

    See Abreview

参考文献 (140)

ab10988 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab10988 は 140 報の論文で使用されています。

  • Han MS  et al. A feed-forward regulatory loop in adipose tissue promotes signaling by the hepatokine FGF21. Genes Dev 35:133-146 (2021). PubMed: 33334822
  • Ni Q  et al. Proper mTORC1 Activity Is Required for Glucose Sensing and Early Adaptation in Human Pancreatic ß Cells. J Clin Endocrinol Metab 106:e562-e572 (2021). PubMed: 33120423
  • Werneck-de-Castro JP  et al. The RNA-binding protein LARP1 is dispensable for pancreatic ß-cell function and mass. Sci Rep 11:2079 (2021). PubMed: 33483593
  • Soltani A  et al. Treatment of diabetic mice by microfluidic system-assisted transplantation of stem cells-derived insulin-producing cells transduced with miRNA. Life Sci 274:119338 (2021). PubMed: 33716064
  • Ramirez-Hernandez G  et al. Global Deletion of the Prolactin Receptor Aggravates Streptozotocin-Induced Diabetes in Mice. Front Endocrinol (Lausanne) 12:619696 (2021). PubMed: 33746901
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 19 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucagon antibody [K79bB10]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Pancreas)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: EDTA
Permeabilization
No
Specification
Pancreas
Fixative
Formaldehyde
Read More

Han-Byul Lee

Verified customer

投稿 May 30 2019

Immunocytochemistry abreview for Anti-Glucagon antibody [K79bB10]

 Good
Abreviews
Application
Immunocytochemistry
Sample
Human Cultured Cells (AD_MSC)
Permeabilization
Yes - 0.25% Triton-X
Specification
AD_MSC
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 26°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Apr 04 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucagon antibody [K79bB10]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Specification
Pancreas
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Formalin
Read More

MISS. Barbara Makela

Verified customer

投稿 Jun 22 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucagon antibody [K79bB10]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (pancreas)
Antigen retrieval step
None
Permeabilization
No
Specification
pancreas
Blocking step
dako antibody diluent with background reducing components as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Jan 03 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucagon antibody [K79bB10]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Syrian Hamster Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 May 29 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucagon antibody [K79bB10]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 May 28 2015

Immunohistochemistry (Frozen sections) abreview for Anti-Glucagon antibody [K79bB10]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (pancreas)
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 May 28 2015

Question

ab87108: ICC/IF image: why is the protein not localized in the nucleus, but rather cytosol or ER?
ab36232: ICC/IF protocol?
ab10988: blocking conditions, ICC/IF protocol?
ab7842: blocking conditions, ICC/IF protocol?

Read More
Answer

Thank you for contacting us.

For ab10988: ICC/IF was added based on published references and a customer Abreview. Thus, a protocol would be based on these sources. We have not tested it ourselves in this application.

The abreview information can be found here. This is all the information we have:
https://www.abcam.com/Glucagon-antibody-K79bB10-ab10988.html&tab=abreviews&intabreviewid=10554

And the references are as follows:

1) Lu J et al. Alpha cell-specific Men1 ablation triggers the transdifferentiation of glucagon-expressing cells and insulinoma development. Gastroenterology 138:1954-65 (2010). ICC/IF, IHC-P; Mouse. PubMed: 20138042

2) Hanley S & Rosenberg L Transforming growth factor beta is a critical regulator of adult human islet plasticity. Mol Endocrinol 21:1467-77 (2007). ICC/IF; Human. PubMed: 17405902

I have not yet heard back yet from the lab regading the other antibodies, but will let you know as soon asI do.

Thank you for your patience.

I hope this information is of some help so far. In the meantime, please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Anti-Glucagon antibody [K79bB10] (ab10988) Dose this antibody have an immunoreactivity with GLP-1 protein?

Read More
Answer

Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you in regards to the Anti-Glucagon antibody [K79bB10] (ab10988).

Glucagon is cleaved into 8 peptide chains one of them is GLP1. As this anhtibody was raised against the polymerised porcine glucagon I cannot say wether the anitbody will detect the GLP1 portion of the protein in particular. I would suggest if you are interested in detecting the GLP1 protein, you may want to consider the following antibodies directed against GLP1:

https://www.abcam.com/Search?Keywords=GLP1&fReset=1&pt=-1&source=TopSearch&btnSearch=Search#Search?AppliedFacets.FacetProductType=Primary+antibodies&AppliedFacets.FacetTargetName=GLP1&IsFacetsOnly=False&PageNumber=1&Keywords%255B0%255D=GLP1&X-Requested-With=XMLHttpRequest

I hope this information has been helpful. If you have any further questions, please do not hesitate to let me know.

Read More

Question

ab87108: ICC/IF image: why is the protein not localized in the nucleus, but rather cytosol or ER?
ab36232: ICC/IF protocol?
ab10988: blocking conditions, ICC/IF protocol?
ab7842: blocking conditions, ICC/IF protocol?

Read More
Answer

Thank you for contacting us.

I have contacted the lab regarding the 4 antibodies you had inquired about, and will be in touch with more informaton as soon as I have it.

Thank you for your patience!


Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

1-10 of 19 Abreviews or Q&A

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