Anti-Glucagon 抗体 [EPR3070-45] (ab108426)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3070-45] to Glucagon
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Glucagon antibody [EPR3070-45]
Glucagon 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3070-45] to Glucagon -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Glucagon recombinant protein; Human pancreas tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 3.31 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3070-45 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108426の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Predicted molecular weight: 21 kDa.
For unpurified, use 1/2000. |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
特記事項 |
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WB
1/5000. Predicted molecular weight: 21 kDa. For unpurified, use 1/2000. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
ターゲット情報
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関連性
Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 2641 Human
- Omim: 138030 Human
- SwissProt: P01275 Human
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別名
- GCG antibody
画像
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Immunohistochemical staining of paraffin embedded human pancreas with purified ab108426 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Unpurified ab108426, at 1/100 dilution, staining Glucagon in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
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Anti-Glucagon antibody [EPR3070-45] (ab108426) at 1/2000 dilution (unpurified) + Glucagon recombinant protein at 10 µg
Predicted band size: 21 kDa -
Unpurified ab108426 showing negative staining in Skeletal muscle tissue.
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Unpurified ab108426 showing negative staining in Normal lung tissue.
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Unpurified ab108426 showing negative staining in Normal kidney tissue.
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Unpurified ab108426 showing negative staining in Normal brain tissue.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab108426 は論文での使用が確認できていません。