Anti-GLP-1 抗体 (ab22625)

Thank you for your enquiry.

I can confirm that this particular productis sourced externally, and regrettably they do not have any specific data or information regarding in house testing for cross reactivity with analogue liraglutide (Lys(γ-Glu-palmitoyl)26,Arg34)-GLP-1 (7-37)). We aim to provide as much information as possible to customers, and I am sorry for the inconvenience on this occasion.

The best informationwe have is provided on the datasheet, and there are also the following references for the various forms of GLP-1 which I hope may be helpful to you.

If you have any further questions, please do not hesitate to contact us.


1. Varndell I.M. et al. Localisation of glucagon-like peptide (GLP) immunoreactants in human gut and pancreas using light and electron
microscopic immunocytochemistry. J. Histochem. Cytochem. 33: 1080-1086, 1985.

2. Kreymann B. et al. Characterisation of glucagon-like peptide-1[7-36] amide in the hypothalamus. Brain Res., 502: 325-331, 1989.

3. Kanse S.M. et al. Identification and characterisation of glucagon-like peptide-1[7-36]amide binding sites in the rat brain and lung. FEBS Lett.,
241: 209-212, 1988.

4. Alvarez, E. et al. Expression of the glucagon-like peptide-1 receptor gene in rat brain. J. Neurochem., 66: 920-927, 1996.

5. Oka, J.I., Goto, N. and Kameyama, T. Glucagon-like peptide-1 modulates neuronal activity in the rat’s hippocampus. Neuroreport, 10: 1643-
1646, 1999.

6. Tateishi K. et al. Glucagon-like peptide-1 (GLP-1) molecular forms in human pancreatic endocrine tumours resemble those in intestine rather
than pancreas. Diabetes Res. Clin. Pract., 25: 43-49, 1994.

7. Drucker, D.J. Glucagon-like peptides. Diabetes 47: 159-169, 1998.

8. Naslund, E. et al. GLP-1 slows solid gastric emptying and inhibits insulin, glucagon, and PYY release in humans. Am. J. Physiol., 277: R910-
R916, 1999.

9. Gutzwiller, J.-P. et al. Glucagon-like peptide-1 promotes satiety and reduces food intake in patients with diabetes mellitus type 2. Am. J.
Physiol., 276: R1541-R1544, 1999. [GA1178 citation]

10. Elahi D. et al. The insulinotropic actions of glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1[7-37] in normal and
diabetic subjects. Regul. Peptides, 51: 63-74, 1994.

11. Fehmann H.C. et al. Glucagon-like peptide-1. Eine therapeutische alternative bei diabetes mellitus? Deutsche Med. Wochensch., 119: 275-
279, 1994.

12. Turton M.D. et al. A role for glucagon-like peptide-1 (GLP-1) in the central regulation of feeding. Nature, 379: 69-72, 1996.

13. Goke R. et al. Exendin-4 is a high potency agonist and truncated exendin[9-39]amide is an antagonist at the glucagon-like peptide-1[7-
36]amide receptor of insulin-secreting beta-cells. J. Biol. Chem., 268: 19650-19655, 1993.

14. Van Dijk G. et al. Glucagon-like peptide-1 and satiety. Nature, 385: 214, 1997.

15. Neary, N.M. et al. Peptide YY3-36 and glucagon-like peptide-17-36 inhibit food intake additively. Endocrinology, Sep 8 2005 (epub).

16. O'Shea D. et al. A role for central glucagon-like peptide-1 in temperature regulation. NeuroReport, 7: 830-832, 1996.

Thank you for contacting us.

ab26278 would work with Mouse tissue and in IHC-Fr. Please be advised that this is a primary antibody not the secondary antibody. The compatible FITC conjugated secondary antibodies are

ab97239, ab98692 and ab115588.

"Cat # 1072-1002" this is not an Abcam catalogue number so please recheck it.

For mouse on mouse staining, please follow the guidelines given inbelow link;

https://www.abcam.com/ps/pdf/protocols/mouse_on_mouse.pdf

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry.

I am sorry I am not able to find catalog number 1072-1002 on our system. Our product numbers always start with ab. We do have the following goat anti mouse IgG FITC conjugated secondary antibody, tested in IHC-Fr, which should be suitable for use with ab26278.

ab7064Goat anti-Mouse IgG H&L (FITC) secondary antibody
FITC conjugated
Flow Cyt, IHC-P, ELISA, IM, ICC/IF, IHC-Fr
https://www.abcam.com/Goat-Mouse-IgG-HL-FITC-preadsorbed-ab7064.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-FITC-preadsorbed-ab7064.html).

I would suggest to bear in mind that the excitation and emission spectra for FITC are very similar to Alexa 488, and so I would not advise using these two conjugates together in duel staining as you will not be able to distinguish between them.

If you would prefer to use a suitable secondary antibody with a different fluorophore, then I can suggest considering the following which has been tested in IHC-Fr:

ab7058Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (TRITC ), pre-adsorbed
TRITC conjugated
https://www.abcam.com/Donkey-Mouse-IgG-HL-TRITC-preadsorbed-ab7058.html (or use the following: https://www.abcam.com/Donkey-Mouse-IgG-HL-TRITC-preadsorbed-ab7058.html).

I can recommend to review the flourophores you wish to use, and if you require any further help please let me know.

This particular secondary (ab7058) is also cross absorbed, which means it is less likely to cross react. However, as you mention, there is always the possibility of crossreactivity when an anti-mouse secondary is used on mouse tissue. I can recommend to review the following page from our website which provides some useful tips:

https://www.abcam.com/index.html?pageconfig=resource&rid=11465

I hope this will be helpful to you. If you have any further questions, please do not hesitate tocontact us.

Thats great news. I have generated a discount code for you:

DISCOUNT CODE: xxxxx
Expiration date: 2012-06-19

As discussed this code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for optimal projection tomography (we don't currently have this as an option on the system but if you choose "whole mount" instead please)and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site:

https://www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests whatever they may be. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscount

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If I can be of any help in optimising your experiments once you get started please do let me know. I look forward to hearing how you get on.

Until then,I wish you all the best with your research.

Thank you for that extra information.

Having gone through the protocol and the additional information about the technique you have provided, I would suggest trying anti-GLP1 antibody, https://www.abcam.com/GLP1-antibody-ab22625.html. This is a rabbit polyclonal to GLP1 which has been used to detect both mouse and human GLP1 protein.I think it is the most likely to work out of the antibodies we have against thistarget as it has been used successfully in immunohistochemistry with frozen sections, paraffin embedded sections and in immunocytochemistry.

As discussed over the phone, I am able to offer you a testing discount for this(or any other anti-GLP1 antibody which you prefer). This would involve you purchasing the antibody, testing it with optimal projection tomography and sharing the results with us through an abreview. Whether the results are positive or negative, you would then be entitled to a free primary antibody of your choice from our catalogue. More information on this scheme can be found from the following link:

https://www.abcam.com/collaboratordiscount

The only stipulation is that you submit and use the code within 4 months of purchase of the initial antibody.

If you would like to participate in this scheme please do let me know and I will issue you with the discount code.

I look forward to receiving your reply.