Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Rat Tissue sections (DRG)
Specification
DRG
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250
Additional data
Additional Notes
A moderate positivity is seen in some ganglion cell bodies.
There is a light positivity in collagen at lower dilutions this is pronounced.
Abcam response
Thank you for your valuable feedback. We were able to find evidence of Glo1 mRNA expression in the rat spinal cord, but were unable to verify protein distribution in the dorsal root ganglion. We welcome further customer comments. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Aug 22 2013