Anti-Gli1 抗体 [EPR4523] (ab134906)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4523] to Gli1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Gli1 antibody [EPR4523]
Gli1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4523] to Gli1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Gli1 aa 1-100. The exact sequence is proprietary.
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ポジティブ・コントロール
- RMS13 and A673 cell lysates; Human brain and testis tissues; A673 cells.
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特記事項
This antibody was developed as part of a collaboration with the lab of Martin Fernandez-Zapico at the Mayo Clinic.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4523 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab134906の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/100 - 1/1000. Predicted molecular weight: 118 kDa.
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特記事項 |
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WB
1/100 - 1/1000. Predicted molecular weight: 118 kDa. |
ターゲット情報
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機能
Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. -
組織特異性
Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. -
配列類似性
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
翻訳後修飾
Phosphorylated in vitro by ULK3. -
細胞内局在
Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1. - Information by UniProt
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参照データベース
- Entrez Gene: 2735 Human
- Omim: 165220 Human
- SwissProt: P08151 Human
- Unigene: 632702 Human
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別名
- Gli 1 antibody
- GLI antibody
- GLI family zinc finger 1 antibody
see all
画像
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All lanes : Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution
Lane 1 : RMS 13 (human rhabdomyosarcoma fibroblast) transfected with scrambled siRNA control whole cell lysate
Lane 2 : RMS 13 (human rhabdomyosarcoma fibroblast) transfected with siRNA specifically targeting Gli1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsDiluting and blocking buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate.
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All lanes : Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution
Lane 1 : A673 (human muscle Ewing's Sarcoma) whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 3 : RD (Human muscle rhabdomyosarcoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsDiluting and blocking buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate.
The expression level of Gli1 in HepG2 and RD are consistent with describes in PMID: 19189974 and PMID: 32493277.
Negative control: HepG2 and RD (PMID: 19189974 and PMID: 32493277).
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Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (purified) + RMS13 cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 118 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (unpurified)
Lane 1 : RMS13 cell lysate
Lane 2 : A673 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
Predicted band size: 118 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab134906 は 30 報の論文で使用されています。
- Kotulova J et al. 2‑Cl‑IB‑MECA regulates the proliferative and drug resistance pathways, and facilitates chemosensitivity in pancreatic and liver cancer cell lines. Int J Mol Med 49:N/A (2022). PubMed: 35039871
- Cui X et al. Collagen type IV alpha 1 (COL4A1) silence hampers the invasion, migration and epithelial-mesenchymal transition (EMT) of gastric cancer cells through blocking Hedgehog signaling pathway. Bioengineered 13:8972-8981 (2022). PubMed: 35297303
- Zhu Y et al. METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition. Cell Death Dis 13:358 (2022). PubMed: 35436987
- Zhao J et al. CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma. J Transl Med 20:520 (2022). PubMed: 36348351
- Zhang S et al. The PROTAC selectively degrading Bcl-xL represents a novel Hedgehog pathway inhibitor with capacity of combating resistance to Smoothened inhibitors while sparing bone growth. Theranostics 12:7476-7490 (2022). PubMed: 36438482