Anti-GITR 抗体 [CAL61] (ab237725)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL61] to GITR
- Suitable for: WB, Flow Cyt (Intra), IHC-P
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-GITR antibody [CAL61]
GITR 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [CAL61] to GITR -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra), IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide within Mouse GITR aa 175-228. The exact sequence is proprietary.
Database link: O35714 -
ポジティブ・コントロール
- IHC-P: Mouse lung and spleen tissue. WB: Mouse splenocytes treated with concanavalin A. Flow Cyt (intra): Mouse splenocytes treated with concanavalin A.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purity >99% -
ポリ/モノ
モノクローナル -
クローン名
CAL61 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab237725の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 26 kDa.
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Flow Cyt (Intra) |
1/500.
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IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 26 kDa. |
Flow Cyt (Intra)
1/500. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. -
組織特異性
Expressed in lymph node, peripheral blood leukocytes and weakly in spleen. -
配列類似性
Contains 3 TNFR-Cys repeats. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 21936 Mouse
- SwissProt: O35714 Mouse
- Unigene: 482508 Mouse
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別名
- Activation inducible TNFR family receptor antibody
- Activation-inducible TNFR family receptor antibody
- AITR antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling GITR with ab237725 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse lung is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GITR with ab237725 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse spleen is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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All lanes : Anti-GITR antibody [CAL61] (ab237725) at 1/1000 dilution
Lane 1 : Untreated mouse splenocytes whole cell lysate
Lane 2 : Mouse splenocytes treated with 2.5µg/ml concanavalin A for 3 days whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDa
Exposure time: 3 minutes33 kDa band represents GITR isoform B based on Uniprot and literature. (PMID: 10836847).
Blocking/Dilution buffer: 5% NFDM/TBST.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed,0.1% Tween-20permeabilized mouse splenocytes treated with 2.5µg/ml concanavalin A for 3 days labeling GITR with ab237725 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD25 conjugated to BV421. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG (Left) or ab237725 (Right).
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Formalin-fixed, paraffin-embedded mouse spleen tissue stained for GITR using ab237725 at 0.125 µg/ml dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab237725 は論文での使用が確認できていません。