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Anti-Ghrelin 抗体 [EPR20502] - BSA and Azide free (ab271988)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Frozen sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20502] to Ghrelin - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-Ghrelin antibody [EPR20502] - BSA and Azide free
    Ghrelin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20502] to Ghrelin - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human, mouse and rat stomach tissues. IHC-Fr: Mouse stomach tissue.

  • 特記事項

    ab271988 is the carrier-free version of ab209790. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-Fr
Mouse
IHC-P
Human
アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).This antibody worked well on mouse IHC-Frozen sections. Staining on rat sections was weak and human samples were not tested.

 
IHC-P
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).This antibody worked well on mouse IHC-Frozen sections. Staining on rat sections was weak and human samples were not tested.

 
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.

  • 組織特異性

    Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.

  • 配列類似性

    Belongs to the motilin family.

  • 翻訳後修飾

    O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Appetite regulating hormone antibody
    • Ghrelin 27 antibody
    • Ghrelin 28 antibody
    • Ghrelin and obestatin prepropeptide antibody
    • Ghrelin antibody
    • Ghrelin/obestatin prepropeptide antibody
    • ghrl antibody
    • GHRL_HUMAN antibody
    • Growth hormone releasing peptide antibody
    • Growth hormone secretagogue antibody
    • Growth hormone-releasing peptide antibody
    • M46 protein antibody
    • Motilin related peptide antibody
    • Motilin-related peptide antibody
    • MTLRP antibody
    • Obestatin antibody
    • Protein M46 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of human stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on human liver [PMID: 10604470].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on mouse liver [PMID: 10604470].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of rat stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on rat liver [PMID: 10604470].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Frozen sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Frozen sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling Ghrelin with ab209790 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID: 18474938]. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This antibody worked well on mouse IHC-Frozen sections. Staining on rat sections was weak and human samples were not tested.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab271988 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab271988 は論文での使用が確認できていません。

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