Anti-GGT7 抗体 (ab272563)

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Western blot - Anti-GGT7 antibody (ab272563)
  • Western blot - Anti-GGT7 antibody (ab272563)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT7 antibody (ab272563)

Key features and details

  • Rabbit polyclonal to GGT7
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-GGT7 antibody

  • 製品の詳細

    Rabbit polyclonal to GGT7

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment corresponding to Human GGT7 aa 350-550.
    Database link: Q9UJ14

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: NIH/3T3, NBT-II, RT4 and U-251 MG cell lysate. IHC-P: Human liver tissue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
WB
Mouse
Human
All applications
Cow
アプリケーション Abreviews 特記事項
WB
Use a concentration of 0.04 - 0.4 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
Use a concentration of 0.04 - 0.4 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性

    GGT7 (Gamma-glutamyltransferase 7) is a member of a gene family that encodes enzymes involved in both the metabolism of glutathione and in the transpeptidation of amino acids. Changes in the activity of gamma glutamyltransferase may signal preneoplastic or toxic conditions in the liver or kidney. GGT7 consists of a heavy and a light chain, and it can interact with CT120, a plasma membrane associated protein that is possibly involved in lung carcinogenesis.

  • 細胞内局在

    Membrane; Single pass type II membrane protein.

  • 参照データベース

    • 別名

      • D20S101 antibody
      • dJ18C9.2 antibody
      • Gamma glutamyltransferase 4 antibody
      • Gamma glutamyltransferase 7 antibody
      • Gamma glutamyltransferase like 3 antibody
      • Gamma glutamyltransferase like 5 antibody
      • Gamma glutamyltranspeptidase 7 antibody
      • Gamma glutamyltranspeptidase like 3 antibody
      • GGT 7 antibody
      • GGT4 antibody
      • GGTL3 antibody
      • GGTL5 antibody
      see all

    画像

    • Western blot - Anti-GGT7 antibody (ab272563)
      Western blot - Anti-GGT7 antibody (ab272563)
      Lanes 2-3 : Anti-GGT7 antibody (ab272563) at 0.4 µg/ml

      Lane 1 : Molecular weight ladder
      Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
      Lane 3 : NBT-II (Rat bladder carcinoma cell line)
    • Western blot - Anti-GGT7 antibody (ab272563)
      Western blot - Anti-GGT7 antibody (ab272563)
      Lanes 2-3 : Anti-GGT7 antibody (ab272563) at 0.4 µg/ml

      Lane 1 : Molecular weight ladder
      Lane 2 : RT4 (Human urinary bladder cancer cell line) cell lysate
      Lane 3 : U-251 MG (Human brain glioma cell line) cell lysate
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT7 antibody (ab272563)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT7 antibody (ab272563)

      Paraffin-embedded formalin-fixed human liver tissue stained for GGT7 using ab272563 at 1/200 in immunohistochemical analysis.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272563 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272563 は論文での使用が確認できていません。

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