Anti-GFR alpha 3/GFRA3 抗体 (ab8028)
Key features and details
- Rabbit polyclonal to GFR alpha 3/GFRA3
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
-
製品名
Anti-GFR alpha 3/GFRA3 antibody
GFR alpha 3/GFRA3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GFR alpha 3/GFRA3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab6100) -
特記事項
Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
DEAE-Chromatography -
一次抗体 備考
Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8028の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml.
|
|
| WB |
1/500 - 1/1000. Predicted molecular weight: 43 kDa.Can be blocked with GFR alpha 3/GFRA3 peptide (ab6100).
|
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 10 µg/ml. |
|
WB
1/500 - 1/1000. Predicted molecular weight: 43 kDa.Can be blocked with GFR alpha 3/GFRA3 peptide (ab6100). |
ターゲット情報
-
機能
Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. -
組織特異性
Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system. -
配列類似性
Belongs to the GDNFR family. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 14587 Mouse
- SwissProt: O35118 Mouse
- Unigene: 16520 Mouse
-
別名
- GDNF family receptor alpha 3 antibody
- GDNF family receptor alpha-3 antibody
- GDNF receptor alpha-3 antibody
see all
画像
-
Western blot - Anti-GFR alpha 3/GFRA3 antibody (ab8028)All lanes : Anti-GFR alpha 3/GFRA3 antibody (ab8028) at 1/500 dilution
Lane 1 : Crude cell membrane fractions of murine heart
Lane 2 : Crude cell membrane fractions of murine spleen
Lane 3 : Crude cell membrane fractions of murine kidney
Lane 4 : Crude cell membrane fractions of murine liver
Lane 5 : Crude cell membrane fractions of murine brain
Predicted band size: 43 kDa
Observed band size: 43 kDaWestern blot analysis of GFR alpha 3/GFRA3 in crude cell membrane fractions of
murine heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with ab8028 at 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFR alpha 3/GFRA3 antibody (ab8028)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse kidney tissue labeling GFR alpha 3/GFRA3 with Anti-GFR alpha 3/GFRA3 antibody (ab8028) at 5μg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFR alpha 3/GFRA3 antibody (ab8028)ab8028 at 10µg/ml staining GFR alpha 3/GFRA3 in mouse heart tissue by IHC-P.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (6)
ab8028 は 6 報の論文で使用されています。
- Rice FL et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
- Han Y et al. Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression. Cell 173:634-648.e12 (2018). PubMed: 29606356
- Ito Y et al. Identification of targets of tumor suppressor microRNA-34a using a reporter library system. Proc Natl Acad Sci U S A 114:3927-3932 (2017). PubMed: 28356515
- Kawamura K et al. Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors. PLoS One 7:e49328 (2012). IHC - Wholemount ; Human . PubMed: 23152897
- Ceyhan GO et al. The neurotrophic factor artemin promotes pancreatic cancer invasion. Ann Surg 244:274-81 (2006). WB, IHC-P ; Human . PubMed: 16858191
- Serra MP et al. Ret, GFRalpha-1, GFRalpha-2 and GFRalpha-3 receptors in the human hippocampus and fascia dentata. Int J Dev Neurosci 23:425-38 (2005). PubMed: 16002253
