Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart (Cardiac fibroblast))
Permeabilization
No
Specification
Heart (Cardiac fibroblast)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% Normal donkey serum in PBS
Dilution
1/100
Additional data
Additional Notes
The antibody cross-reactivity with YFP was tested by performing immunofluorescence on cardiac frozen sections of hearts from Tcf21MCM:R26Ch2-YFP animals. An excellent overlap with the native YFP signal was detected and hence this antibody can be used to detect YFP.
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投稿 Apr 06 2021