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  1. Link

    gfp-antibody-ab6673.pdf

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Tags & Cell Markers Fusion / Marker Proteins GFP
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Anti-GFP 抗体 (ab6673)

  • Datasheet
  • SDS
Reviews (20)Q&A (6)References (310)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry - Free Floating - Anti-GFP antibody (ab6673)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab6673)
  • Immunohistochemistry (Frozen sections) - Anti-GFP antibody (ab6673)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab6673)
  • Western blot - Anti-GFP antibody (ab6673)
  • Immunofluorescence - Anti-GFP antibody (ab6673)
  • Western blot - Anti-GFP antibody (ab6673)
  • Western blot - Anti-GFP antibody (ab6673)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab6673)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab6673)

Key features and details

  • Goat polyclonal to GFP
  • Suitable for: WB, IP, ELISA, ICC/IF, IHC-P, IHC-FrFl, IHC-Fr
  • Reacts with: Species independent
  • Isotype: IgG

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Anti-Podoplanin / gp36 antibody [8.1.1] (ab92319)

関連製品

製品の概要

  • 製品名

    Anti-GFP antibody
    GFP 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to GFP
  • 由来種

    Goat
  • 特異性

    Anti-GFP assayed by ELISA for direct binding of antigen recognizes wild type, recombinant and enhanced forms of GFP.
  • アプリケーション

    適用あり: WB, IP, ELISA, ICC/IF, IHC-P, IHC-FrFl, IHC-Frmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Fusion protein corresponding to Aequorea victoria GFP aa 1-246.
    Database link: P42212

  • ポジティブ・コントロール

    • IHC: E5.5 Hex-GFP transgenic mouse embryo. WB: Pure GFP protein, or cells known to overexpress GFP.
  • 特記事項

    This anti-GFP antibody cross reacts with eGFP .

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 精製度

    Affinity purified
  • 特記事項(精製)

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GFP

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant E. coli GFP protein (His tag) (ab119740)
    • Recombinant A. victoria GFP protein (ab84191)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • GFP ELISA Kit (ab171581)

アプリケーション

Our Abpromise guarantee covers the use of ab6673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/400 - 1/2000.

(for immunoprecipitated GFP, see Abreview).

IP Use at an assay dependent concentration.
ELISA 1/40000.

This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP.

ICC/IF 1/500.
IHC-P 1/200 - 1/1000.
IHC-FrFl Use at an assay dependent concentration.
IHC-Fr 1/200 - 1/1000.
IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • 別名

    • GFP antibody
    • Green fluorescent protein antibody

画像

  • Immunohistochemistry - Free Floating - Anti-GFP antibody (ab6673)
    Immunohistochemistry - Free Floating - Anti-GFP antibody (ab6673)Suarez-Bregua et al PLoS One. 2017 Oct 17;12(10):e0186444. doi: 10.1371/journal.pone.0186444. eCollection 2017. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Pth4:eGFP transgenic zebrafish embryos at 1 and 2 dpf were fixed with 4% PFA and washed in PBST. They were then washed in PBDT (1% BSA, 1% DMSO, 0.1% Triton X-100 in PBS, pH 7.4), blocked in 10% normal goat serum/PBDT, and incubated overnight at 4°C with primary antibodies to HuC/D (1/100) and GFP (1/400, Abcam ab6673). Further PBST washes and blocking were followed by secondary antibodies overnight at 4°C. Hoechst 34580 was added to stain nuclei (1/2500). After further PBDT and PBS washes, embryos were mounted for confocal imaging.

    Abbreviation: e, eye; hy, hypothalamus; m, midbrain; sc, spinal cord. Scale bars: 100 μm (A-C) 50 μm (D-G).

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab6673)
    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab6673)Borkowska et al PLoS One. 2016 May 31;11(5):e0156082. doi: 10.1371/journal.pone.0156082. eCollection 2016. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    In utero electroporation of Disc1 and Disc1-100P constructs into wild-type neocortex and analysis at P21.

    (Panels D-E”) Expression of the constructs was assessed.

    (Panels D-D'') 2 days after transfection in vitro.

    (Panels E-E'') at P21 in vivo.

    Immunochemistry for FLAG and GFP showed that constructs encoding either WT Disc1, the Disc1-100P variant, or GFP alone, expressed these protein species in transfected HEK-293 cells in vitro (Fig 5D–5D”) and in P21 postmitotic cortical neurons in vivo (Fig 5E–5E”)

  • Immunohistochemistry (Frozen sections) - Anti-GFP antibody (ab6673)
    Immunohistochemistry (Frozen sections) - Anti-GFP antibody (ab6673)Goldman et al PLoS One. 2018 Jan 12;13(1):e0191245. doi: 10.1371/journal.pone.0191245. eCollection 2018. Fig 5. Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

    Immunofluorescence for assessment of GFP+ myofibers in rat tissue.

    VML affected muscle from the 50% MG + HA+LMN group were probed for the presence of GFP. GFP+ fibers were detected in a qualitatively similar magnitude at both 2 and 8 weeks post-injury indicating viable engraftment of donor derived muscle progenitor cells. Scale bars are 1mm for whole mount images, 50 μm for regions of interest.

    A portion of the TA muscle from the defect region was embedded in a talcum-based gel, frozen in 2-methylbutane, and supercooled in liquid nitrogen. Cryosections (8 μm) were prepared and stained using standard protocols for hematoxylin & eosin.

    ab6673 used at a 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab6673)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab6673)Cedeno et al PLoS One. 2017 Sep 21;12(9):e0185196. doi: 10.1371/journal.pone.0185196. eCollection 2017. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Mouse small intestines were washed with DPBS and fixed overnight at 4°C in Zinc formalin. Following sectioning and tissue deparaffanization, antigen retrieval was performed with 10mM Tris base (pH 9.0) buffer using a pressure cooker.

    For immunohistochemistry, sections were quenched of endogenous peroxidases by 3% H2O2, and sequentially blocked with Avidin D, biotin, and protein blocking reagents. Primary antibody incubation was conducted at 4°C overnight. Secondary biotinylated antibody was added at a dilution of 1/200, and incubated 2 hours at room temperature. Finally, sections were stained according to the ABC peroxidase protocol and counterstained with hematoxylin.

    ab6673 used at a 1/200 dilution.

    Panel D: Representative anti-eGFP immunofluorescence of macroH2A WT and DKO jejunum counterstained with DAPI (blue).

  • Western blot - Anti-GFP antibody (ab6673)
    Western blot - Anti-GFP antibody (ab6673)
    All lanes : Anti-GFP antibody (ab6673) at 1 µg/ml (o/n at 4degC)

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) lysate at 10 µg
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 10 µg
    Lane 3 : CHO/K1 lysate at 10 µg
    Lane 4 : MDA-MB-231 (Human breast adenocarcinoma cell line) lysate at 10 µg
    Lane 5 : A431 (Human epidermoid carcinoma cell line) lysate at 10 µg
    Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) lysate at 10 µg
    Lane 7 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate
    Lane 8 : E-coli HCP control, 50 ng
    Lane 9 : FLAG Positive control lysate at 10 µg
    Lane 10 : Red fluorescent protein, 50 ng
    Lane 11 : Green fluorescent protein, 50 ng
    Lane 12 : Glutathinoe-S-Transferase protein, 50 ng
    Lane 13 : Maltose Binding protein, 50 ng

    Secondary
    All lanes : Peroxidase goat secondary antibody, 60 min at RT at 1/30000 dilution


    Blocking Buffer: 1% Casein-TTBS for 30 min at RT.

  • Immunofluorescence - Anti-GFP antibody (ab6673)
    Immunofluorescence - Anti-GFP antibody (ab6673)

    E5.5 Hex-GFP transgenic mouse embryo stained for GFP using ab6673 at 1/500 dilution. Secondary antibody is a fluorochrome conjugated anti-goat IgG secondary antibody at 1/10,000 for 45 min at RT.

    Staining: GFP as green fluorescent signal with DAPI blue counterstain.

  • Western blot - Anti-GFP antibody (ab6673)
    Western blot - Anti-GFP antibody (ab6673)
    All lanes : Anti-GFP antibody (ab6673) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cells
    Lane 2 : Mock transfected HeLa cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : IRDye® 800 conjugated Donkey-a-Goat IgG [H&L] at 1/2500 dilution

    Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-GFP antibody (ab6673)
    Western blot - Anti-GFP antibody (ab6673)This image is courtesy of an anonymous abreview.
    All lanes : Anti-GFP antibody (ab6673) at 1/1000 dilution

    Lane 1 : MRC5VA lung fibroblast whole cell lysate overexpressing EGFP alone
    Lanes 2-3 : MRC5VA lung fibroblast whole cell lysate overexpressing an EGFP fusion protein

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-goat polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 27,55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab6673)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab6673)This image is courtesy of Bart Rountree

    Immunofluorescence of TGN mouse liver labeling GFP on hepatocytes with ab6673.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab6673)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab6673)This image is courtesy of Jeff Klein

    Immunohistochemistry of GFP transgenic mouse liver labeling GFP with ab6673. 

プロトコール

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (310)

    ab6673 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6673 は 310 報の論文で使用されています。

    • Xu T  et al. Myofibroblast induces hepatocyte-to-ductal metaplasia via laminin-?vß6 integrin in liver fibrosis. Cell Death Dis 11:199 (2020). PubMed: 32251270
    • Zieger HL  et al. Disease-associated synaptic scaffold protein CNK2 modulates PSD size and influences localisation of the regulatory kinase TNIK. Sci Rep 10:5709 (2020). PubMed: 32235845
    • Chen L  et al. The role of bone marrow-derived cells in the origin of liver cancer revealed by single-cell sequencing. Cancer Biol Med 17:142-153 (2020). PubMed: 32296582
    • Morales Torres C  et al. Selective inhibition of cancer cell self-renewal through a Quisinostat-histone H1.0 axis. Nat Commun 11:1792 (2020). PubMed: 32286289
    • Klepfish M  et al. LOXL2 Inhibition Paves the Way for Macrophage-Mediated Collagen Degradation in Liver Fibrosis. Front Immunol 11:480 (2020). PubMed: 32296422
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 26 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
    Sample
    Mouse Tissue sections (spleen, red pulp, hematopoietic tissue)
    Specification
    spleen, red pulp, hematopoietic tissue
    Permeabilization
    Yes - Tween-20
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 01 2014

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (Brain, Neural Stem Cells)
    Specification
    Brain, Neural Stem Cells
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Miss. Katya Zelentsova

    Verified customer

    投稿 Feb 26 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (embryo)
    Permeabilization
    Yes - Tritonx100
    Specification
    embryo
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 29 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brainstem)
    Antigen retrieval step
    None
    Specification
    brainstem
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 23 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Uterus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium citrate
    Permeabilization
    Yes - Tween-20
    Specification
    Uterus
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Reshef Tal

    Verified customer

    投稿 Sep 06 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-GFP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (testis)
    Permeabilization
    Yes - 0.04% Tween20
    Specification
    testis
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 28 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (GFP labelled 4T1 cells in the lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ph9
    Permeabilization
    No
    Specification
    GFP labelled 4T1 cells in the lung
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Dr. Victoria Bridgeman

    Verified customer

    投稿 May 27 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (esophagus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM citric acid, pH6.0
    Specification
    esophagus
    Blocking step
    Non-Abcam blocking agent as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Tatiana Karakasheva

    Verified customer

    投稿 Feb 22 2016

    Question

    Does this antibody cross-react with other GFP variants?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 01 2015

    Answer

    This anti GFP antibody has reacted with all tested variants of GFP from A. Victoria tested including YFP. We have not tested with eYFP specifically. The lots we have tested have not shown cross reactivity to RFP.

    Read More

    Heather Allen

    Abcam Scientific Support

    Answered on Oct 01 2015

    IHC - Wholemount abreview for Anti-GFP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Epidermal sheet of the skin)
    Specification
    Epidermal sheet of the skin
    Read More

    Miss. yaara tabib

    Verified customer

    投稿 Jun 19 2014

    1-10 of 26 Abreviews or Q&A

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    製品・研究分野別
    • 癌
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    • 発生
    • エピジェネティクス&核内情報
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