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  1. Link

    gfp-antibody-ab6556.pdf

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Tags & Cell Markers Fusion / Marker Proteins GFP
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Anti-GFP 抗体 (ab6556)

  • Datasheet
  • SDS
Reviews (61)Q&A (22)References (1073)

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Abpromise

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Immunocytochemistry - Anti-GFP antibody (ab6556)
  • Immunocytochemistry - Anti-GFP antibody (ab6556)
  • Electron Microscopy - Anti-GFP antibody (ab6556)

Key features and details

  • Rabbit polyclonal to GFP
  • Suitable for: IHC-P, Electron Microscopy, ICC, IP, Flow Cyt, IHC-Fr, WB
  • Reacts with: Species independent
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-GFP antibody [EPR14104] (ab183734)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-GFP antibody
    GFP 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to GFP
  • 由来種

    Rabbit
  • 特異性

    GFP antibody (ab6556) is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.

  • アプリケーション

    適用あり: IHC-P, Electron Microscopy, ICC, IP, Flow Cyt, IHC-Fr, WBmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Recombinant full length protein corresponding to GFP.
    Database link: P42212

  • 特記事項

    Please note that a mistake was made in reference 4 (Mesaeli et.al., J. Cell. Biol. 1999 Mar 8;144(5):857-68). The antibody used for immunohistochemistry on paraformaldehyde fixed tissues was the crude serum version of this antibody (Abcam ab290) and not Clontech's monoclonal as stated. This product is supplied in 25% glycerol. During freezeing and thawing some phase separation might occur - Please ensure that the solution is mixed thoroughly but GENTLY before use.

    This antibody (ab6556) is the purified version of our best-selling rabbit polyclonal to GFP (ab290). It has been developed specifically for use in applications requiring a high titre and specificity with minimum background such as immuno-electron microscopy.

    This anti-GFP antibody recognizes the enhanced form of GFP as well.

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Constituents: 0.79% Tris HCl, 25% Glycerol
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    This antibody is an affinity purified rabbit anti-GFP antibody purified on an affinity chromatography column made with highly purified recombinant GFP.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GFP

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HEK-293 whole cell lysate (ab7902)
  • Recombinant Protein

    • Recombinant E. coli GFP protein (His tag) (ab119740)
    • Recombinant A. victoria GFP protein (ab84191)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • GFP ELISA Kit (ab171581)
    • Anti-GFP antibody (ab290)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6556の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (11)
Use at an assay dependent concentration.
Electron Microscopy
1/5000.
ICC (1)
1/2000.
IP (3)
Use at an assay dependent concentration.
Flow Cyt (1)
Use at an assay dependent concentration.
IHC-Fr (4)
Use at an assay dependent concentration.
WB (27)
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
特記事項
IHC-P
Use at an assay dependent concentration.
Electron Microscopy
1/5000.
ICC
1/2000.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.

ターゲット情報

  • 関連性

    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • 別名

    • GFP antibody
    • Green fluorescent protein antibody

画像

  • Immunocytochemistry - Anti-GFP antibody (ab6556)
    Immunocytochemistry - Anti-GFP antibody (ab6556)

    This image shows a single primary hippocampal neuron from a primary culture overexpressing GFP stained with ab6556 at a dilution of 1/2000. This picture was kindly supplied as part of the review submitted by one of our customers.

  • Immunocytochemistry - Anti-GFP antibody (ab6556)
    Immunocytochemistry - Anti-GFP antibody (ab6556)

    This image shows IF using GFP-expressing glial cells (green) transplanted into lesioned rat spinal cord. This was detected using ab6556 anti-GFP antibody and a FITC conjugated secondary antibody. Axons are labelled red by an antibody to neurofilament-200 and a rhodamine secondary. ab6556 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious. The top picture shows an optical section from a confocal microscope scan showing how a GFP cell wraps around a branched axon travelling longitudinally. The bottom picture consists of an optical section from another confocal scan showing a GFP cell enveloping an axon in the transverse plane. Review by Andrew Toft submitted 19 May 2004.

  • Electron Microscopy - Anti-GFP antibody (ab6556)
    Electron Microscopy - Anti-GFP antibody (ab6556)

    Specific labeling of a Trk-GFP fusion protein being synthesized on ER in sympathetic neurons infected with an adenovirus carrying the construct. The gold is associated with the ER membranes. This was done using a 1/5000 dilution of affinity purified antibody (ab6556). The tissue section was fixed and embedded using durcupan resin.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (1073)

ab6556 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6556 は 1073 報の論文で使用されています。

  • Montenegro-Venegas C  et al. Bassoon inhibits proteasome activity via interaction with PSMB4. Cell Mol Life Sci 78:1545-1563 (2021). PubMed: 32651614
  • Wei H  et al. Clock component OsPRR73 positively regulates rice salt tolerance by modulating OsHKT2;1-mediated sodium homeostasis. EMBO J 40:e105086 (2021). PubMed: 33347628
  • Mukai K  et al. Homeostatic regulation of STING by retrograde membrane traffic to the ER. Nat Commun 12:61 (2021). PubMed: 33397928
  • Phatak V  et al. Mutant p53 promotes RCP-dependent chemoresistance coinciding with increased delivery of P-glycoprotein to the plasma membrane. Cell Death Dis 12:207 (2021). PubMed: 33627632
  • González-Calvo I  et al. Sushi domain-containing protein 4 controls synaptic plasticity and motor learning. Elife 10:N/A (2021). PubMed: 33661101
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 83 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 30 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MC3T3 cells)
Permeabilization
Yes - NP40
Specification
MC3T3 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Jan 11 2021

Western blot abreview for Anti-GFP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
Transfected to express EGFP
Specification
U2OS
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 Dec 16 2020

Western blot abreview for Anti-GFP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MC3T3 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
Transfected to express EGFP
Specification
MC3T3 cells
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 Dec 16 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
Yes - Tween-20
Specification
Uterus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Reshef Tal

Verified customer

投稿 Aug 30 2019

Western blot abreview for Anti-GFP antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
15 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Feb 06 2019

Western blot abreview for Anti-GFP antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
HEK 293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Apr 23 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (lung)
Permeabilization
No
Specification
lung
Blocking step
Blockaid as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 May 31 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (fat)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH6.0
Permeabilization
No
Specification
fat
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

DR. Jiang Hu

Verified customer

投稿 Mar 16 2017

Question

We are aiming to perform a double immunohistochemistry against eGFP and jRED. Do your anti GFP antibodies (https://www.abcam.com/GFP-antibody-ab290.html, https://www.abcam.com/GFP-antibody-ab13970.html, https://www.abcam.com/GFP-antibody-ab6556.html) cross react with jRED protein? Anti-jRED antibody does not cross react with (Evrogen, AB961) with GFP.

Read More

Abcam community

Verified customer

Asked on May 21 2012

Answer

Based on the sequence homology, I do not think that the jRED antibodies would cross react with GFP and vice versa.

Read More

Abcam Scientific Support

Answered on May 21 2012

1-10 of 83 Abreviews or Q&A

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