Application
Western blot
Sample
Mouse Cell lysate - nuclear (mouse neurons)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
mouse neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye 800
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
LiCor
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 37 kDa
Additional data
Additional Notes
Transduction of mouse neurons with empty vector or GFP fusion protein lentivirus.
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投稿 Jan 20 2022