Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.1% Triton in PBS (5 mins)
Specification
HeLa
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
ab290 greatly enhanced fluorescence of fixed HeLa cells transfected with GFP-POLG2 as most of the natural fluorescence was quenched by fixation.
A mitochondrial antibody was used in conjunction to show correct localisation.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 19 2021