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Tags & Cell Markers Fusion / Marker Proteins GFP
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Anti-GFP 抗体 (ab290)

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  • SDS
Reviews (173)Q&A (90)References (2234)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)
  • Immunohistochemistry - Free Floating - Anti-GFP antibody (ab290)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)
  • Immunocytochemistry - Anti-GFP antibody (ab290)
  • Immunoprecipitation - Anti-GFP antibody (ab290)
  • Immunocytochemistry - Anti-GFP antibody (ab290)
  • Immunoprecipitation - Anti-GFP antibody (ab290)
  • Immunocytochemistry - Anti-GFP antibody (ab290)
  • Western blot - Anti-GFP antibody (ab290)
  • Western blot - Anti-GFP antibody (ab290)
  • Western blot - Anti-GFP antibody (ab290)

Key features and details

  • Rabbit polyclonal to GFP
  • Suitable for: ELISA, IHC-FrFl, Electron Microscopy, IHC-FoFr, ICC, IHC-P, IHC-Fr, IP, WB
  • Reacts with: Species independent
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-GFP antibody [EPR14104] (ab183734)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-GFP antibody
    GFP 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to GFP
  • 由来種

    Rabbit
  • 特異性

    Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin. In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.

    This antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP and EGFP.

     

  • アプリケーション

    適用あり: ELISA, IHC-FrFl, Electron Microscopy, IHC-FoFr, ICC, IHC-P, IHC-Fr, IP, WBmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Recombinant full length protein corresponding to GFP.
    Database link: P42212

  • 特記事項

    The total IgG concentration has been determined to be 5 mg/mL. The specific IgG concentration is unknown. This product should be kept refrigerated at all times whilst in short term storage. Using sterilised equipment will reduce the risk of bacterial contamination. 

    Anti-GFP antibody (ab6556) is the purified version of this antibody (see Related Products).

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: 1.25% Sodium chloride
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • 特記事項(精製)

    This antibody is provided as whole antiserum. It is not possible to determine the exact antibody concentration, since whole serum contains many other host serum proteins besides the antibody of interest.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GFP

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (FITC) (ab6717)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant A. victoria GFP protein (ab84191)
  • Related Products

    • GFP ELISA Kit (ab171581)
    • Anti-GFP antibody (ab6556)

アプリケーション

Our Abpromise guarantee covers the use of ab290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration.
Electron Microscopy 1/1000 - 1/4000.
IHC-FoFr 1/200 - 1/500.
ICC 1/200 - 1/1000.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) secondary antibody.

IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Reported to work at dilutions up to 1/3000. Use secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077).

IP Use at an assay dependent concentration. Use at 1µl per 10cm tissue culture dish (use 10µl protein A agarose CL4B to precipitate the immune complex).
WB 1/1000 - 1/2500.

It is recommended to use 12.5% SDS-PAGE and to transfer to PVDF membrane. Use 1x Blotto (or 3% BSA in PBS) for diluting and blocking. Use PBS in 3x 5min washing steps throughout the immunolabelling. Probe with ab290 at 1:1000 - 1:5000 dilution and use Goat Anti-Rabbit IgG H&L (HRP) (ab205718) at 1:5000 dilution with ECL detection method. ab290 has been reported to work at 1:50,000 and dilutions around this range should be tested if high background is seen. Both incubation steps should be for 1hr at 22°C.

ターゲット情報

  • 関連性

    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • 別名

    • GFP antibody
    • Green fluorescent protein antibody

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)Image from Yang C et al., PLoS One. 2013;8(11):e79615. Fig 2.; doi:10.1371/journal.pone.0079615. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Bone marrow-derived infiltrating cells in the stromal tissue of gastric intraepithelial tumor traced by GFP direct fluorescence.

    (A) Normal tissues of the glandular stomach of a regular GFP(−) control mouse. (B) Normal tissues of the glandular stomach of a GFP(+) transgenic control mouse; (C, E, D, F) An induced gastric intraepithelial neoplasia (GIN) in a bone marrow transplanted mouse. GFP(+) BMDCs tracked with direct fluorescence localized in the GIN stromal tissue are shown in C and E. The same GIN lesion slide stained by H&E after the fluorescence observation are shown in D and F. DAPI (A–C and E) and hematoxylin (D and F) are used to visualize nuclei, respectively. Locations of the images C and D in the images E and F, and the image E in the image F are marked in the corresponding color. The gastric glands and stromal cells are also labeled.

  • Immunohistochemistry - Free Floating - Anti-GFP antibody (ab290)
    Immunohistochemistry - Free Floating - Anti-GFP antibody (ab290)This image is courtesy of an Abreview submitted by Judith Kranz

    Immunohistochemistry (Free Floating) analysis of mouse brain tissue sections labelling GFP with ab290. Tissue was fixed with 4% PFA, frozen 30 µm sections were blocked for 1 hour at room temperature with 10% normal goat serum + donkey anti-mouse IgG Fab fragments (0.1 mg/ml). Sections were incubated with the primary antibody at a dilution of 1/1000 in TBS + 0.25% Triton-X for 16 hours at 4°C. A Cy2®-conjugated donkey anti-rabbit IgG (H+L) at a dilution of 1/200 was used as the secondary antibody.

    Image shows anti-NeuN (red), DAPI (blue), and anti-GFP staining of GFP-cre (green, yellow with NeuN colocalization).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)This image is courtesy of an anonymous Abreview

    ab290 staining dog hearts (Adv-GFP injection) tissue sections by IHC-P.  Sections were PFA fixed and subjected to heat mediated antigen retrieval in citric acid (Ph6.0, 0.05% Tween20) prior to blocking with 10% serum for 30 mins at 37°C.  The primary antibody was diluted 1/1000 in PBS and incubated with the sample for 1 hour at 25°C.  A HRP conjugated secondary like  Goat Anti-Rabbit IgG H&L (HRP) (ab205718) was used.

    See Abreview

  • Immunocytochemistry - Anti-GFP antibody (ab290)
    Immunocytochemistry - Anti-GFP antibody (ab290)

    Immunofluorescence images showing similar localization of Yes-GFP (first 10 aa's of Yes PTK fused to the N-terminus of GFP) to full length Yes PTK. A: Distribution of Yes detected using mouse anti-Yes Ab followed by Texas Red-conjugated anti-mouse Ab. B: Chimeric GFP's detected using rabbit anti-GFP Ab (Abcam ab290) followed by FITC-conjugated anti-rabbit Ab.

    Image kindly provided by L.G. Berthiaume. Taken from J. McCabe and L.G. Berthiaume, Functional Roles for Fatty Acylated Amino-terminal Domains in Subcellular Localization, Molecular Biology of the Cell 10:3771-3786, 1999

  • Immunoprecipitation - Anti-GFP antibody (ab290)
    Immunoprecipitation - Anti-GFP antibody (ab290)This image is courtesy of an Abreview submitted by William Hung

    ab290 immunoprecipitating GFP in HEK293 nuclear lysate expressing GFP. 20µg of lysate was incubated with primary antibody (1 µg/mg lysate) and matrix (Protein G) for 16 hours at 4°C in AFC low salt buffer. For western blotting ab290 (1/5000) was used to confirm successful immunoprecipation.

    Lane 1: HEK293 nuclear lysate expressing GFP input.
    Lane 2: IP of HEK293 nuclear lysate expressing GFP.
    Lane 3: Cells with no GFP.

    See Abreview

  • Immunocytochemistry - Anti-GFP antibody (ab290)
    Immunocytochemistry - Anti-GFP antibody (ab290)

    ab290 staining GFP in GFP-transfected NIH3T3 cells. The cells were fixed with 4% formaldehyde (10min) and then blocked in 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab290 at 1/200 dilution overnight at +4°C followed by incubation with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081), for 1 hour, at 1μg/ml.

    Under identical experimental conditions, when compared to the basal level of GFP expression in transfected NIH3T3 cells, the cells upon which ab290 was applied gave a stronger signal in the 488 channel, indicating that ab290 is binding to GFP and therefore eliciting signal amplification.

    ab290 was also applied to non-GFP-transfected NIH3T3 cells, which produced no positive staining, indicating specificity for GFP. Nuclear DNA was labelled with 1.43μM DAPI (blue).

  • Immunoprecipitation - Anti-GFP antibody (ab290)
    Immunoprecipitation - Anti-GFP antibody (ab290)This image is courtesy of an Abreview submitted by Vladimir Milenkovic

    ab290 immunoprecipitate in human HEK293 cells transfected with Annexin1-GFP. 25µg of cell lysate was incubated with the primary antibody and matrix (Protein G) in 1% TX-100, 10% glycerol, 1X PBS for 16 hours at 4°C. For Western blotting anti-rabbit HRP conjugated secondary antibody was used at a dilution at 1/5000.

    Lane 1: Lysate of HEK293 cells expressing Annexin1-GFP fusion protein.
    Lane 2: IP with anti-GFP.
    Lane 3: Not bound fraction.

    See Abreview

  • Immunocytochemistry - Anti-GFP antibody (ab290)
    Immunocytochemistry - Anti-GFP antibody (ab290)This image is courtesy of an anonymous Abreview

    ab290 staining GFP in U2OS cells expressing TRF2-GFP fusion protein by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with NP40 and blocked with 3% BSA for 1 hour at 21°C. Samples were incubated with the primary antibody (1/1000 in PBS + 3% BSA) for 12 hours at 4°C. An Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at a dilution of 1/500 was used as the secondary antibody.

    Green - GFP.
    Blue - DAPI.

    See Abreview

  • Western blot - Anti-GFP antibody (ab290)
    Western blot - Anti-GFP antibody (ab290)
    Anti-GFP antibody (ab290) at 1/2500 dilution + Recombinant A. victoria GFP protein (ab84191) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 27 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Secondary antibody - goat anti-rabbit HRP preadsorbed (ab97080)

  • Western blot - Anti-GFP antibody (ab290)
    Western blot - Anti-GFP antibody (ab290)This image is courtesy of an anonymous Abreview
    All lanes : Anti-GFP antibody (ab290) at 1/5000 dilution

    Lane 1 : LNCaP whole cell lysate - pEGFP empty vector
    Lane 2 : LNCaP whole cell lysate - pEGFP-PKD1 transfected

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds


    Blocked with 5% milk for 1 hour at 23°C.

    Incubated with the primary antibody for 16 hours at 4°C.

    See Abreview

  • Western blot - Anti-GFP antibody (ab290)
    Western blot - Anti-GFP antibody (ab290)This image is courtesy of an Abreview submitted by S Houtman
    All lanes : Anti-GFP antibody (ab290) at 1/5000 dilution

    Lane 1 : COS7 whole cell lysate - transfected with GFP-Eml4
    Lane 2 : COS7 whole cell lysate - transfected with GFP

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated pig anti-rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 30 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocked with 5% milk for 1 hour at 20°C.

    Incubated with the primary antibody for 18 hours at 4°C in TBS containing 2% milk and 1% Tween.

    Predicted MW of Eml4 ~ 120 kDa.

    See Abreview

プロトコール

  • Immunocytochemistry/ Immunofluorescence

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (2234)

    ab290 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290 は 2234 報の論文で使用されています。

    • Logan SM & Benson MD Medial epithelial seam cell migration during palatal fusion. J Cell Physiol 235:1417-1424 (2020). PubMed: 31264714
    • Zhou YQ  et al. Genetically engineered distal airway stem cell transplantation protects mice from pulmonary infection. EMBO Mol Med 12:e10233 (2020). PubMed: 31782624
    • Hose J  et al. The genetic basis of aneuploidy tolerance in wild yeast. Elife 9:N/A (2020). PubMed: 31909711
    • Su P  et al. High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy. Mol Brain 13:5 (2020). PubMed: 31931837
    • Mei H  et al. Increased mitochondrial DNA copy number protects hair cells and HEI-OC1 cells against drug-induced apoptosis. Mol Med Rep 21:338-346 (2020). PubMed: 31939628
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 263 Abreviews or Q&A

    Western blot abreview for Anti-GFP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Recombinant protein (HEK293T cells over expressing mouse 5-HT6R)
    Loading amount
    50 µg
    Specification
    HEK293T cells over expressing mouse 5-HT6R
    Gel Running Conditions
    Reduced Denaturing (8% Tris-Glycine gel)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 21 2013

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFP antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. J. Y. Seo

    Verified customer

    投稿 Mar 27 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Colon)
    Specification
    Colon
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 12 2013

    Question

    Does your antibody anti-GFP ab290 also recognize turbo-GFP?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 19 2013

    Answer

    To our knowledge the detection of turbo-GFP has not been tested with ab290. Turbo-GFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata , which is a different organism that the classical/original GFP which is from aquorea victoria jelly fish. The Aequorea victoria GFP (http://www.uniprot.org/uniprot/P42212) only has 26% homology with copepod GFP (http://www.uniprot.org/uniprot/Q6WV12), so we would not expect ab290 to recognize turbo-GPF or cop-GFP.

    Read More

    Abcam Scientific Support

    Answered on Feb 19 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2oS)
    Permeabilization
    Yes - 0.1% Tween
    Specification
    U2oS
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 06 2012

    Western blot abreview for Anti-GFP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    5 µg
    Specification
    HEK293
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 19 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-GFP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain (eGFP-GFAP transgenic mice))
    Permeabilization
    No
    Specification
    Brain (eGFP-GFAP transgenic mice)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 05 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    投稿 Jul 06 2020

    Western blot abreview for Anti-GFP antibody - ChIP Grade

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver and brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    50 µg
    Treatment
    GFP transduced
    Specification
    liver and brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 04 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Calvaria bone regeneration with GFP labeled cells)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
    Permeabilization
    No
    Specification
    Calvaria bone regeneration with GFP labeled cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Dr. Queralt Vallmajó

    Verified customer

    投稿 Mar 19 2020

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