Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (GFP-expressing Astrocytes in Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
GFP-expressing Astrocytes in Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Dilution
1/25
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: anti Mouse IgG Biotin conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Under these conditions I get no positivity in Astrocytes expressing GFP.
Submitted composite image shows this: serial sections were immunostained with this Ab ( upper image) and ab13970 ( lower image).
Arrowheads: black for same cell showing in both sections, red for blood vessel appearing in both sections, green for immunostaining of endogenous IgG in residual serum of capillary ( a MOM reaction).
Abcam response
Thank you for submitting an Abreview. IHC-P was not a tested application for ab1218. ab1218 apparently does not seem to work well in this experimental procedure, under these conditions. Thank you for your time, this information will surely be helpful for customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Jan 06 2012