Application
Western blot
Sample
Human Cell lysate - whole cell (HL-60, Jurkat, Raw264.7, PC12)
Loading amount
20 µg
Specification
HL-60, Jurkat, Raw264.7, PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% Milk PBS-Tween 20 (0.1%)
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti Rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
Supersignal West Dura
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 48-52 kDa
Additional data
Additional Notes
Lane order: HL-60 (lane 1), Jurkat (lane 2), Raw264.7 (lane 3), PC12 (lane 4)
Detects a band of 48-50 kDa in all lysates tested. A faint band migrating at ~52 kDa was observed in several of the lysates. Strong signal was observed in both rodent lines (lanes 3 & 4)
Detects a band of 48-50 kDa in all lysates tested. A faint band migrating at ~52 kDa was observed in several of the lysates. Strong signal was observed in both rodent lines (lanes 3 & 4)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
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投稿 Apr 04 2008