Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen retrieval: Citrate buffer 1x 3 min en olla a presiòn 150¹ (preparado a partir de 10x Heat mediated antigen retrieval solution pH:6 - 250 ml abcam ref ab973)
Sample
Mouse Tissue sections (retina with diabetic retinopathy (positive GFAP))
Specification
retina with diabetic retinopathy (positive GFAP)
Permeabilization
Yes - PBS-triton x-100 0,025%
Fixative
Methanol
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: anti GFAP ab 33922 diluted in 1% BSA/TBS
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: ALEXA 488 GOAT ANTI RABBIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
I work a lot of years with antibody GFAP ab7260 with positive results. In this case, I want to prove ab33922 because is RabMab with high homology (>85%) between rat and mouse). I expected as better results for visualize the endfeet of Mûller cells in the diabetic retina.
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 10 2015