Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Brain)
Permeabilization
Yes - 0.1% Triton X100
Specification
Brain
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: sea block
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Abcam antibody:Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed
Additional data
Additional Notes
GFAP positive cells are pseudo colored in Green and the nuclei are in Blue using Hoechst stain in this rat brain culture.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 31 2019