Application
IHC - Wholemount
Sample
Mouse Tissue (Brain)
Specification
Brain
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Phosphate buffer with 0.1% Triton X-100
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-chicken
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Tissue was perfusion fixed in 4% paraformaldehyde in sodium cacodylate buffer (pH: 7.4) for 15 mins at room temperature and subsequently immersion fixed overnight at 4 degrees Celsius. Tissue was then sectioned at a 100um using a vibratome. Anti-GFAP (green).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Gabriel Luna
Verified customer
投稿 Jan 05 2018