Application
Flow Cytometry
Sample
Human Cell (Brain Cell)
Specification
Brain Cell
Preparation
Cell harvesting/tissue preparation method: Collected using 0.05% trypsin and trypsin inhibitor.
Sample buffer: Cells were grown in stem cell media, RHB-A.
Sample buffer: Cells were grown in stem cell media, RHB-A.
Fixation
Acetone
Permeabilization
No
Gating Strategy
Cells expressing GFAP
Other product details
Dilution
1/100
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: 10% FCS in PBS
Secondary antibody
Name
Abcam antibody:Goat anti-Chicken IgY H&L (FITC) secondary antibody
Dilution
1/100
Additional data
Additional Notes
Cells were fixed in 80% acetone for 5 -10 minutes at -20°C. Cells were incubated with primary antibody (1/100) for 20 minutes at 25°C in 10% FCS in PBS. A FITC-conjugated Goat polyclonal to chicken IgY H&L was used as a secondary antibody at 1/200 dilution.
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投稿 May 24 2012