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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments
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Anti-GFAP 抗体 (ab4674)

  • Datasheet
  • SDS
Reviews (55)Q&A (6)References (289)

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Abpromise

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Immunocytochemistry - Anti-GFAP antibody (ab4674)
  • Immunohistochemistry (PFA fixed) - Anti-GFAP antibody (ab4674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab4674)
  • Immunohistochemistry - Free Floating - Anti-GFAP antibody (ab4674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab4674)
  • Western blot - Anti-GFAP antibody (ab4674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab4674)

Key features and details

  • Chicken polyclonal to GFAP
  • Suitable for: IHC (PFA fixed), IHC-FrFl, ICC, IHC-P, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgY

こちらの製品もご検討ください

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関連製品

製品の概要

  • 製品名

    Anti-GFAP antibody
    GFAP 一次抗体 製品一覧
  • 製品の詳細

    Chicken polyclonal to GFAP
  • 由来種

    Chicken
  • アプリケーション

    適用あり: IHC (PFA fixed), IHC-FrFl, ICC, IHC-P, WBmore details
  • 種交差性

    交差種: Mouse, Rat
    交差が予測される動物種: Mammals
  • 免疫原

    Recombinant full length protein corresponding to Human GFAP. Isotype 1 expressed in and purified from E. coli.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • バッファー

    Preservative: 0.03% Sodium azide
  • Concentration information loading...
  • 精製度

    IgY fraction
  • 特記事項(精製)

    Concentrated IgY fraction of egg yolks.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgY
  • 研究分野

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class II
    • GFAP
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Stem Cells
    • Neural Stem Cells
    • Glial Restricted Lineage
    • Astrocyte

関連製品

  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant Human GFAP protein (ab114149)
    • Recombinant Human GFAP protein (ab151370)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Human GFAP Matched Antibody Pair Kit (ab222279)

アプリケーション

Our Abpromise guarantee covers the use of ab4674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC (PFA fixed) Use at an assay dependent concentration.
IHC-FrFl 1/1000 - 1/5000.

Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.

ICC Use a concentration of 1 mg/ml.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa.

Expect to see a band at 55kDa and another at about 48kDa, apparently a breakdown product of the 55kDa band.

ターゲット情報

  • 機能

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 組織特異性

    Expressed in cells lacking fibronectin.
  • 関連疾患

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 配列類似性

    Belongs to the intermediate filament family.
  • 翻訳後修飾

    Phosphorylated by PKN1.
  • 細胞内局在

    Cytoplasm. Associated with intermediate filaments.
  • Target information above from: UniProt accession P14136 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 14580 Mouse
    • Entrez Gene: 24387 Rat
    • SwissProt: P03995 Mouse
    • SwissProt: P47819 Rat
    • Unigene: 1239 Mouse
    • Unigene: 91512 Rat
    • 別名

      • wu:fb34h11 antibody
      • ALXDRD antibody
      • cb345 antibody
      • etID36982.3 antibody
      • FLJ42474 antibody
      • FLJ45472 antibody
      • GFAP antibody
      • GFAP_HUMAN antibody
      • gfapl antibody
      • Glial fibrillary acidic protein antibody
      • Intermediate filament protein antibody
      • wu:fk42c12 antibody
      • xx:af506734 antibody
      • zgc:110485 antibody
      see all

    画像

    • Immunocytochemistry - Anti-GFAP antibody (ab4674)
      Immunocytochemistry - Anti-GFAP antibody (ab4674)

      ab4674 staining GFAP in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4674 at 1µg/ml and ab87122, Rabbit Poly to Mouse Fructose-bisphosphate aldolase C (No Modifications). Cells were then incubated with ab150176, Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in red) and ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in pseudocolour green). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 4% paraformaldehyde (10 min).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Immunohistochemistry (PFA fixed) - Anti-GFAP antibody (ab4674)
      Immunohistochemistry (PFA fixed) - Anti-GFAP antibody (ab4674)

      GFAP antibody ab4674 was used with Tissue Clearing Kit ab243298 to penetrate, stain and clear a 500 μm section of rat brain.

      Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain thick tissue sections and get more data from each valuable tissue section.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab4674)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab4674)Elmore MR. et al PLoS One. 2015 Apr 7;10(4):e0122912. doi: 10.1371/journal.pone.0122912. eCollection 2015. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      10x and 63x z-stack images of the CA1 hippocampal region for each treatment are shown, with NeuN staining in green and GFAP staining in red.

      Two month-old wild-type mice were placed on either control (n = 10) or inhibitor diet (PLX3397, provided at 290 mg/kg chow; n = 14) for 21 d, causing the elimination of approximately 99% of microglia brain-wide.

      Fluorescent immunolabeling of the microglia followed a standard indirect technique (primary antibody followed by fluorescent secondary antibody). Brain tissue (sliced at 40 μm) was stained using the anti-ionized calcium-binding adapter molecule 1 (IBA1, polyclonal, rabbit) antibody (1:1000; Wako, Cat. #019–19741), mounted on slides, and coverslipped using Dapi Fluoromount-G (SouthernBiotech). Half brain images were obtained by stitching using a Zeiss AxioImager M2 upright microscope and Stereo Investigator software package from MicroBrightField. In addition, tissue was stained with anti-hexaribonucleotide binding protein-3 (NeuN, monoclonal, mouse) antibody (1:1000; Millipore; Cat. #MAB377) to label neurons and anti-glial fibrillary acidic protein (GFAP, polyclonal, chicken) antibody (1:500; Abcam; Cat. #ab4674) to label astrocytes, and 10x and 63x z-stack images obtained for each treatment using confocal microscopy.

       

    • Immunohistochemistry - Free Floating - Anti-GFAP antibody (ab4674)
      Immunohistochemistry - Free Floating - Anti-GFAP antibody (ab4674)

      Immunofluorescent analysis of a section of mouse hippocampus stained with ab4674 at a 1:5,000 dilution in green.

      Costained with a rabbit pAb to FOX3/NeuN dilution 1:5,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, mouse brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained. The GFAP antibody stains a network of astroglial cells while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab4674)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab4674)

      IHC image of GFAP staining in a formalin-fixed, paraffin-embedded mouse normal brain tissue section.

      The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6). The section was incubated with ab4674 at 1/1000 dilution for 15 minutes at room temperature. A goat anti-chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

    • Western blot - Anti-GFAP antibody (ab4674)
      Western blot - Anti-GFAP antibody (ab4674)
      All lanes : Anti-GFAP antibody (ab4674) at 1/5000 dilution

      Lane 1 : Rat whole brain lysate
      Lane 2 : Mouse whole brain lysate

      Predicted band size: 50 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab4674)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab4674)

      IHC image of GFAP staining in a formalin fixed, paraffin embedded normal rat hippocampus tissue section.

      The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6). The section was incubated with ab4674 at 1/1000 dilution for 15 minutes at room temperature. A goat anti-chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

    プロトコール

    • Immunostaining of Cells in Tissue Culture

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (289)

    ab4674 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4674 は 289 報の論文で使用されています。

    • Linker KE  et al. Microglial activation increases cocaine self-administration following adolescent nicotine exposure. Nat Commun 11:306 (2020). PubMed: 31949158
    • Skórkowska A  et al. Effect of Combined Prenatal and Adult Benzophenone-3 Dermal Exposure on Factors Regulating Neurodegenerative Processes, Blood Hormone Levels, and Hematological Parameters in Female Rats. Neurotox Res 37:683-701 (2020). PubMed: 31970650
    • Wang J  et al. A New Rat Model of Chronic Cerebral Hypoperfusion Resulting in Early-Stage Vascular Cognitive Impairment. Front Aging Neurosci 12:86 (2020). PubMed: 32351379
    • Stevenson TJ  et al. a-synuclein inclusions are abundant in non-neuronal cells in the anterior olfactory nucleus of the Parkinson's disease olfactory bulb. Sci Rep 10:6682 (2020). PubMed: 32317654
    • Gee MS  et al. A selective p38a/ß MAPK inhibitor alleviates neuropathology and cognitive impairment, and modulates microglia function in 5XFAD mouse. Alzheimers Res Ther 12:45 (2020). PubMed: 32317025
    View all Publications for this product

    レビューと Q&A

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    1-10 of 61 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-GFAP antibody

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    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Brain)
    Specification
    Brain
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    Mr. Gabriel Luna

    Verified customer

    投稿 Jan 05 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFAP antibody

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    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 4°C
    Antigen retrieval step
    None
    Sample
    Rabbit Tissue sections (eye)
    Specification
    eye
    Permeabilization
    No
    Fixative
    Formaldehyde
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    Dr. Alex Zagariya

    Verified customer

    投稿 Dec 17 2013

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFAP antibody

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    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.5% triton
    Specification
    Brain
    Blocking step
    Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Formalin
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 01 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFAP antibody

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    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Zebrafish Tissue sections (Embryo)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Embryo
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
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    Abcam user community

    Verified customer

    投稿 Jun 24 2020

    Immunohistochemistry free floating abreview for Anti-GFAP antibody

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    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
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    Verified customer

    投稿 Jun 22 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFAP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
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    Abcam user community

    Verified customer

    投稿 Jun 09 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFAP antibody

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    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - triton 1%
    Specification
    brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Francoise Geffroy

    Verified customer

    投稿 May 28 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-GFAP antibody

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    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Lizard Tissue sections (Anolis carolinensis Tail Spinal Cord Cross-Section)
    Permeabilization
    Yes - 0.1% Triton X-100/TBS for 10 min at room temperature
    Specification
    Anolis carolinensis Tail Spinal Cord Cross-Section
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
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    Dr. Megan Hudnall

    Verified customer

    投稿 Mar 24 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody

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    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brain cell culture)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Brain cell culture
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 18 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFAP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Brain
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 11 2020

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