Anti-GFAP 抗体 (ab16997)
Key features and details
- Rabbit polyclonal to GFAP
- Suitable for: ICC, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GFAP antibody
GFAP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GFAP -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Full length native protein (purified) corresponding to Cow GFAP. Isolated from cow spinal cord.
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特記事項
In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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精製度
IgG fraction -
一次抗体 備考
In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16997の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC |
1/100.
|
|
IHC-P | (1) |
1/50 - 1/250.
|
特記事項 |
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ICC
1/100. |
IHC-P
1/50 - 1/250. |
ターゲット情報
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機能
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
組織特異性
Expressed in cells lacking fibronectin. -
関連疾患
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylated by PKN1. -
細胞内局在
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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参照データベース
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- Unigene: 514227 Human
- Unigene: 1239 Mouse
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別名
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
画像
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue, staining GFAP with ab16997.
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Mouse cortical primary astrocytes stained for GFAP using ab16997 at a dilution of 1/100 in ICC/IF.
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Formalin-fixed, paraffin-embedded section of human brain stained with antibodies to GFAP. Cytoplasmic staining of glia is shown in red.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (57)
ab16997 は 57 報の論文で使用されています。
- Pietiläinen O et al. Astrocytic cell adhesion genes linked to schizophrenia correlate with synaptic programs in neurons. Cell Rep 42:111988 (2023). PubMed: 36640364
- Yan J et al. Met-RANTES preserves the blood-brain barrier through inhibiting CCR1/SRC/Rac1 pathway after intracerebral hemorrhage in mice. Fluids Barriers CNS 19:7 (2022). PubMed: 35062973
- Curreli S et al. Complementary encoding of spatial information in hippocampal astrocytes. PLoS Biol 20:e3001530 (2022). PubMed: 35239646
- Molnár K et al. Motoneuronal inflammasome activation triggers excessive neuroinflammation and impedes regeneration after sciatic nerve injury. J Neuroinflammation 19:68 (2022). PubMed: 35305649
- Mehlhorn J et al. Regional Patterning of Adult Neurogenesis in the Homing Pigeon's Brain. Front Psychol 13:889001 (2022). PubMed: 35898980