Application
Immunocytochemistry
Sample
Mouse Cell (cultured neurones)
Specification
cultured neurones
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5%
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgG Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Host species: Goat
Clonality: Polyclonal
Additional data
Additional Notes
I used this antibody at 1/200 and 1/500 in standard ICC for 1h at RT and neither dilution worked. I also incubated the antibody at 1/100 overnight at 4?C and it still didn't work. I would suggest using another abcam anti-GFAP [GF5] (ab10062 ) which worked fine in these preparations and conditions using the same secondary antibody.
Abcam response
Thank you for testing this antibody on mouse cells and for sharing your results with us. Originally, ab8975 has been tested and characterized on human cells and it seems that it does not recognize mouse GFAP. We believe this information will be very useful for our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Randal Moldrich
Verified customer
投稿 Dec 20 2005