Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RTP°C
Sample
Mouse Cell (Mouse primary astrocyte)
Specification
Mouse primary astrocyte
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/T
Dilution
1/500
Additional data
Additional Notes
The ab4648 antibody did not work in primary mouse astrocytes at 1:500. No fluorescence was detected at all. We ran this alongside ab80842, which worked beautifully also at 1:500 (overnight).
Abcam response
Thank you for your valuable feedback. Scientific support were contacted and we issued the customer with ab80842 as a replacement.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Craig Beall
Verified customer
投稿 Sep 15 2014