Anti-Geminin 抗体 (ab12147)
Key features and details
- Rabbit polyclonal to Geminin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Geminin antibody
Geminin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Geminin -
由来種
Rabbit -
特異性
This antibody detects a band representing Geminin by Western blot at 33kD on 293T cell lysate, this band can be blocked by the immunising peptide. It also detects a band at about 45kD that is not blocked by the immunising peptide and is thought to be non-specific. The immunogen shares low homology with Mouse Geminin, however some customers have reported positive results when testing on mouse. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
非交差種: Mouse -
免疫原
Synthetic peptide corresponding to Human Geminin aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab12408) -
ポジティブ･コントロール
- This antibody gave a positive signal in Hek293 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12147の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
1/500. Detects a band of approximately 33 kDa (predicted molecular weight: 23.5 kDa).
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| 特記事項 |
|---|
|
WB
1/500. Detects a band of approximately 33 kDa (predicted molecular weight: 23.5 kDa). |
ターゲット情報
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機能
Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. -
配列類似性
Belongs to the geminin family. -
発生段階
Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. - Information by UniProt
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参照データベース
- Entrez Gene: 51053 Human
- Omim: 602842 Human
- SwissProt: O75496 Human
- Unigene: 234896 Human
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別名
- DNA replication inhibitor antibody
- Gem antibody
- GEMI_HUMAN antibody
see all
画像
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Western blot - Anti-Geminin antibody (ab12147)
Western blot using ab12147 at 1/500.
Lane 1: 293 lysate
The lower band represents Geminin (33 kDa - predicted molecular weight 23 kDa), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide.
Lane 2: 293 lysate with blocking peptide ab12408.
Lysate at 5µ
The lower band represents Geminin (33kD - predicted molecular weight 23kd), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide. -
Western blot - Anti-Geminin antibody (ab12147)Lane 1 : Anti-Geminin antibody (ab12147) at 1 µg/ml
Lane 2 : Anti-6X His tag® antibody (ab9108) at 1 µg/ml
All lanes : Recombinant Human Geminin protein (ab40570)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 23.5 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
Western blot - Anti-Geminin antibody (ab12147)This image is a courtesy of Anonymous AbreviewAnti-Geminin antibody (ab12147) at 1/500 dilution + Cell lysate prepared from human HeLa nuclear extract at 50 µg
Secondary
HRP-conjugated goat poyclonal to rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23.5 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (11)
ab12147 は 11 報の論文で使用されています。
- Wasserman D et al. Cell cycle oscillators underlying orderly proteolysis of E2F8. Mol Biol Cell 31:725-740 (2020). PubMed: 31995441
- Ryan D et al. Targeting AXL and RAGE to prevent geminin overexpression-induced triple-negative breast cancer metastasis. Sci Rep 9:19150 (2019). PubMed: 31844158
- Zargoun IM et al. DNA ploidy and cell cycle protein expression in oral squamous cell carcinomas with and without lymph node metastases. J Oral Pathol Med 46:738-743 (2017). PubMed: 28135012
- Tan J & Martin SE Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening. PLoS One 11:e0168968 (2016). PubMed: 28030641
- Zhang S et al. lncRNA Up-Regulated in Nonmuscle Invasive Bladder Cancer Facilitates Tumor Growth and Acts as a Negative Prognostic Factor of Recurrence. J Urol 196:1270-8 (2016). PubMed: 27267320
