Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cells)
Specification
Hela cells
Fixative
Methanol
Permeabilization
No
Blocking step
Fish scale gelatin in PBS as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: 0.2 % Fish scale gelatin in PBS
Secondary antibody
Name
Non-Abcam antibody was used: donkey anti-mouse IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Gemin 2 is clearly visualized (green channel) in the cytoplasm, and weakly in nuclei of Hela cells by immunostaining using Anti-Gemin 2 antibody [2E17] (ab6084).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 24 2012