Anti-Gemin 2 抗体 [2E17] (ab6084)
Key features and details
- Mouse monoclonal [2E17] to Gemin 2
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human, Xenopus laevis
- Isotype: IgG1
製品の概要
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製品名
Anti-Gemin 2 antibody [2E17]
Gemin 2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2E17] to Gemin 2 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human, Xenopus laevis -
免疫原
His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine).
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特記事項
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from supernatant. -
一次抗体 備考
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5. -
ポリ/モノ
モノクローナル -
クローン名
2E17 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab6084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
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機能
The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. -
配列類似性
Belongs to the gemin-2 family. -
細胞内局在
Nucleus > gem. Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 8487 Human
- Omim: 602595 Human
- SwissProt: O14893 Human
- SwissProt: O42260 Xenopus laevis
- Unigene: 652307 Human
- Unigene: 3305 Xenopus laevis
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別名
- Component of gems 2 antibody
- Gem (nuclear organelle) associated protein 2 antibody
- Gem associated protein 2 antibody
see all
画像
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![Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)](https://www.abcam.co.jp/ps/products/6/ab6084/Images/ab6084-12015-anti-gemin-2-antibody-2e17-flow-cytometry.jpg)
Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunocytochemistry/ Immunofluorescence - Anti-Gemin 2 antibody [2E17] (ab6084)](https://www.abcam.co.jp/ps/products/6/ab6084/Images/ab6084-12014-anti-gemin-2-antibody-2e17-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Gemin 2 antibody [2E17] (ab6084)Image from Morency E et al, J Cell Biol. 2007 Jun 4;177(5):757-68, Fig 4f. DOI 10.1083/jcb.200612107.HeLa cells were transfected with an ICP0-expressing plasmid before IF assays to detect the distribution of CB- and gem-associated proteins. ab6084 used at a 1/200 dilution to detect Gemin 2 (green). Bar 5 µm.
プロトコール
参考文献 (8)
ab6084 は 8 報の論文で使用されています。
- Staropoli JF et al. Rescue of gene-expression changes in an induced mouse model of spinal muscular atrophy by an antisense oligonucleotide that promotes inclusion of SMN2 exon 7. Genomics 105:220-8 (2015). PubMed: 25645699
- Groen EJ et al. ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN. Hum Mol Genet N/A:N/A (2013). PubMed: 23681068
- Sanchez G et al. A novel function for the survival motoneuron protein as a translational regulator. Hum Mol Genet : (2012). PubMed: 23136128
- Wu CY et al. Proteomic assessment of a cell model of spinal muscular atrophy. BMC Neurosci 12:25 (2011). WB ; Mouse . PubMed: 21385431
- Burnett BG et al. Regulation of SMN protein stability. Mol Cell Biol 29:1107-15 (2009). WB ; Human . PubMed: 19103745
- Walker MP et al. SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain. Hum Mol Genet 17:3399-410 (2008). ICC/IF ; Mouse . PubMed: 18689355
- Morency E et al. A novel cell response triggered by interphase centromere structural instability. J Cell Biol 177:757-68 (2007). ICC/IF ; Human . PubMed: 17548509
- Renvoisé B et al. Distinct domains of the spinal muscular atrophy protein SMN are required for targeting to Cajal bodies in mammalian cells. J Cell Sci 119:680-92 (2006). PubMed: 16449324
